Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers
ABSTRACT: Intestinal inflammation is a primary contributor to poor growth performance during poultry production. Chlorogenic acid (CGA) is a natural phenolic acid that exhibits superior anti-inflammatory activity and improved intestinal health. To investigate the protective effects and molecular mec...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-05-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001652 |
| _version_ | 1797277339088846848 |
|---|---|
| author | Huawei Liu Hongling Meng Mengmeng Du Huimin Lv Yang Wang Kai Zhang |
| author_facet | Huawei Liu Hongling Meng Mengmeng Du Huimin Lv Yang Wang Kai Zhang |
| author_sort | Huawei Liu |
| collection | DOAJ |
| description | ABSTRACT: Intestinal inflammation is a primary contributor to poor growth performance during poultry production. Chlorogenic acid (CGA) is a natural phenolic acid that exhibits superior anti-inflammatory activity and improved intestinal health. To investigate the protective effects and molecular mechanisms of CGA during intestinal inflammation in lipopolysaccharide (LPS)-challenged broilers, we randomly divided 288 one-day-old male Cobb broilers into 4 groups: a control group fed a basal diet (CON group), a basal diet + LPS group (LPS group), and 2 basal diet groups fed 500 or 750 mg/kg CGA + LPS (CGA_500 or CGA_750 groups). Broilers were injected with LPS or saline at 15, 17, 19, and 21 d old. Chlorogenic acid supplementation improved the growth performance of LPS-challenged broilers by increasing average daily gain (ADG) and reducing feed/gain (F/G) ratios (P < 0.05). CGA also improved intestinal barrier function in LPS-challenged boilers by enhancing jejunum morphology and integrity, decreasing intestinal permeability, and increasing occludin 3, zonula occludens-1, and mucin 2 expression (P < 0.05). CGA supplementation also improved systemic and jejunum antioxidant capacity by significantly enhancing glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities (P < 0.05), and reducing malonaldehyde (MDA) and protein carbonyl (PCO) levels (P < 0.05). Chlorogenic acid supplementation reduced systemic and jejunum pro-inflammatory cytokines (interleukin (IL)-1β, IL-6, and IL-12) and increased anti-inflammatory cytokines (IL-10) in LPS-challenged broilers (P < 0.05) by inhibiting the toll like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling pathway (P < 0.05). In addition, the protective effects of CGA toward intestinal inflammation and apoptosis appeared to be correlated with inhibited endoplasmic reticulum (ER) stress (P < 0.05). In summary, CGA supplementation improved intestinal morphology and integrity by inhibiting TLR4/NF-κB and ER stress pathways, which potentially reduced oxidative stress and inflammation, and ultimately improved the growth performance of LPS-challenged broilers. |
| first_indexed | 2024-03-07T15:47:14Z |
| format | Article |
| id | doaj.art-2c9ce7ddd0b24bc99d60b9793f9d1a66 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-07T15:47:14Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-2c9ce7ddd0b24bc99d60b9793f9d1a662024-03-05T04:29:24ZengElsevierPoultry Science0032-57912024-05-011035103586Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilersHuawei Liu0Hongling Meng1Mengmeng Du2Huimin Lv3Yang Wang4Kai Zhang5College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCorresponding author:; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaABSTRACT: Intestinal inflammation is a primary contributor to poor growth performance during poultry production. Chlorogenic acid (CGA) is a natural phenolic acid that exhibits superior anti-inflammatory activity and improved intestinal health. To investigate the protective effects and molecular mechanisms of CGA during intestinal inflammation in lipopolysaccharide (LPS)-challenged broilers, we randomly divided 288 one-day-old male Cobb broilers into 4 groups: a control group fed a basal diet (CON group), a basal diet + LPS group (LPS group), and 2 basal diet groups fed 500 or 750 mg/kg CGA + LPS (CGA_500 or CGA_750 groups). Broilers were injected with LPS or saline at 15, 17, 19, and 21 d old. Chlorogenic acid supplementation improved the growth performance of LPS-challenged broilers by increasing average daily gain (ADG) and reducing feed/gain (F/G) ratios (P < 0.05). CGA also improved intestinal barrier function in LPS-challenged boilers by enhancing jejunum morphology and integrity, decreasing intestinal permeability, and increasing occludin 3, zonula occludens-1, and mucin 2 expression (P < 0.05). CGA supplementation also improved systemic and jejunum antioxidant capacity by significantly enhancing glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities (P < 0.05), and reducing malonaldehyde (MDA) and protein carbonyl (PCO) levels (P < 0.05). Chlorogenic acid supplementation reduced systemic and jejunum pro-inflammatory cytokines (interleukin (IL)-1β, IL-6, and IL-12) and increased anti-inflammatory cytokines (IL-10) in LPS-challenged broilers (P < 0.05) by inhibiting the toll like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling pathway (P < 0.05). In addition, the protective effects of CGA toward intestinal inflammation and apoptosis appeared to be correlated with inhibited endoplasmic reticulum (ER) stress (P < 0.05). In summary, CGA supplementation improved intestinal morphology and integrity by inhibiting TLR4/NF-κB and ER stress pathways, which potentially reduced oxidative stress and inflammation, and ultimately improved the growth performance of LPS-challenged broilers.http://www.sciencedirect.com/science/article/pii/S0032579124001652intestinal inflammatory, broilerchlorogenic acid, NF-κB, endoplasmic reticulum stress |
| spellingShingle | Huawei Liu Hongling Meng Mengmeng Du Huimin Lv Yang Wang Kai Zhang Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers Poultry Science intestinal inflammatory, broiler chlorogenic acid, NF-κB, endoplasmic reticulum stress |
| title | Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers |
| title_full | Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers |
| title_fullStr | Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers |
| title_full_unstemmed | Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers |
| title_short | Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers |
| title_sort | chlorogenic acid ameliorates intestinal inflammation by inhibiting nf κb and endoplasmic reticulum stress in lipopolysaccharide challenged broilers |
| topic | intestinal inflammatory, broiler chlorogenic acid, NF-κB, endoplasmic reticulum stress |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001652 |
| work_keys_str_mv | AT huaweiliu chlorogenicacidamelioratesintestinalinflammationbyinhibitingnfkbandendoplasmicreticulumstressinlipopolysaccharidechallengedbroilers AT honglingmeng chlorogenicacidamelioratesintestinalinflammationbyinhibitingnfkbandendoplasmicreticulumstressinlipopolysaccharidechallengedbroilers AT mengmengdu chlorogenicacidamelioratesintestinalinflammationbyinhibitingnfkbandendoplasmicreticulumstressinlipopolysaccharidechallengedbroilers AT huiminlv chlorogenicacidamelioratesintestinalinflammationbyinhibitingnfkbandendoplasmicreticulumstressinlipopolysaccharidechallengedbroilers AT yangwang chlorogenicacidamelioratesintestinalinflammationbyinhibitingnfkbandendoplasmicreticulumstressinlipopolysaccharidechallengedbroilers AT kaizhang chlorogenicacidamelioratesintestinalinflammationbyinhibitingnfkbandendoplasmicreticulumstressinlipopolysaccharidechallengedbroilers |