Development of a Droplet Digital™ PCR DNA methylation detection and quantification assay of prenatal tobacco exposure

Development of a Droplet Digital™ PCR DNA methylation detection and quantification assay of prenatal tobacco exposure

DNA methylation is a labile modification associated with gene expression control and environmental adaptations. High throughput, scalable and quantitative assessments of specific DNA methylation modifications in complex genomic regions for use in large population studies are needed. The performance...

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Bibliographic Details
Main Authors: Katti Arroyo, Anahit Nargizyan, Francianne G Andrade, Swe Swe Myint, Sabrina Lu, Priyatama Pandey, Amy Yee, Adam J de Smith, Joseph L Wiemels
Format: Article
Language:English
Published: Taylor & Francis Group 2022-04-01
Series:BioTechniques
Subjects:
bisulfite treatment
CpG dense regions
differentially methylated regions
DNA methylation
Droplet Digital™ PCR (ddPCR™)
gBlocks™ Gene Fragments
Online Access:https://www.future-science.com/doi/10.2144/btn-2021-0099

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https://www.future-science.com/doi/10.2144/btn-2021-0099

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