Comparison of mammosphere formation from stem-like cells of normal breast, malignant primary breast tumors, and MCF-7 cell line

Comparison of mammosphere formation from stem-like cells of normal breast, malignant primary breast tumors, and MCF-7 cell line

Abstract Background Mammosphere formation assay has become a versatile tool to quantify the activity of putative breast cancer stem cells in non-adherent in vitro cultures. However, optimizing the suspension culture system is crucial to establish mammosphere cultures from primary breast tumors. Meth...

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Bibliographic Details
Main Authors: Jenifer Mallavarpu Ambrose, Vishnu Priya Veeraraghavan, Rosy Vennila, Secunda Rupert, Jeswanth Sathyanesan, Rajasundari Meenakshisundaram, Sakthivel Selvaraj, Sarubala Malayaperumal, Malathi Kullappan, Sudarsanam Dorairaj, Jayesh R. Gujarathi, Sri Harshini Gandhamaneni, Krishna Mohan Surapaneni
Format: Article
Language:English
Published: SpringerOpen 2022-12-01
Series:Journal of the Egyptian National Cancer Institute
Subjects:
Breast cancer stem-like cells
Breast cancer stem cells
Mammosphere assay
Mammosphere formation
Mammosphere-forming efficiency
Online Access:https://doi.org/10.1186/s43046-022-00152-1

Internet

https://doi.org/10.1186/s43046-022-00152-1

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