An improved procedure for subcellular spatial alignment during live-cell CLEM.

Live-cell correlative light and electron microscopy (CLEM) offers unique insights into the ultrastructure of dynamic cellular processes. A critical and technically challenging part of CLEM is the 3-dimensional relocation of the intracellular region of interest during sample processing. We have devel...

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Bibliographic Details
Main Authors: Benjamin S Padman, Markus Bach, Georg Ramm
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3995996?pdf=render