Engineering and Characterization of 3-Aminotyrosine-Derived Red Fluorescent Variants of Circularly Permutated Green Fluorescent Protein

Engineering and Characterization of 3-Aminotyrosine-Derived Red Fluorescent Variants of Circularly Permutated Green Fluorescent Protein

Introducing 3-aminotyrosine (aY), a noncanonical amino acid (ncAA), into green fluorescent protein (GFP)-like chromophores shows promise for achieving red-shifted fluorescence. However, inconsistent results, including undesired green fluorescent species, hinder the effectiveness of this approach. In...

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Bibliographic Details
Main Authors: Hao Zhang, Xiaodong Tian, Jing Zhang, Hui-wang Ai
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Biosensors
Subjects:
nonconical amino acid
protein engineering
redox sensitivity
red-shifted fluorescence
Online Access:https://www.mdpi.com/2079-6374/14/1/54

Internet

https://www.mdpi.com/2079-6374/14/1/54

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