Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya
The condition of eating places such as street vendors will determine the level of food hygiene, including the water used to wash food utensils. This study aimed to determine the number of microbes and identify Escherichia coli and Salmonella sp. on the water that was used to wash tableware by street...
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Format: | Article |
Language: | English |
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Universitas Islam Negeri Sunan Ampel Surabaya
2021-09-01
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Series: | Biotropic: The Journal of Tropical Biology |
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Online Access: | http://jurnalsaintek.uinsby.ac.id/index.php/biotropic/article/view/1316 |
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author | Fahimatul Ula Misbakhul Munir Hanik Faizah |
author_facet | Fahimatul Ula Misbakhul Munir Hanik Faizah |
author_sort | Fahimatul Ula |
collection | DOAJ |
description | The condition of eating places such as street vendors will determine the level of food hygiene, including the water used to wash food utensils. This study aimed to determine the number of microbes and identify Escherichia coli and Salmonella sp. on the water that was used to wash tableware by street vendors around UIN Sunan Ampel Surabaya. Water samples before and after being used to wash tableware were obtained from 4 street vendors. The number of microbes in the sample was tested by the Total Plate Count (TPC) and Most Probable Number (MPN) methods, Escherichia coli was identified using Eosin Methylene Blue Agar (EMB) media, and Salmonella sp. was identified using Salmonella-Shigella agar (SSA) and Triple Sugar Iron agar (TSIA) media. Based on the results of TPC test, the water sample before being used to wash tableware had the lowest and highest values that were 3.0 x 102 Cfu/ml and 9.8 x 105 Cfu/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 4.0 x 102 Cfu/ml and 3.5 x 105 Cfu/ml, respectively. In the MPN test, the water sample before being used to wash tableware had the lowest and highest values that were 15/100ml and 26 100/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 72/100 ml and 271/ 100 ml, respectively. Escherichia coli was detected in all samples 1&3 while in sample 2 Escherichia coli was detected in the water before it was detected the genus Enterobacter and in samples 4 the genus Enterobacter was detected and all samples water used to wash tableware of street vendors was positive for Salmonella. |
first_indexed | 2024-12-18T00:12:30Z |
format | Article |
id | doaj.art-2d1596f73b684d49946ec3889f21e689 |
institution | Directory Open Access Journal |
issn | 2580-5029 |
language | English |
last_indexed | 2024-12-18T00:12:30Z |
publishDate | 2021-09-01 |
publisher | Universitas Islam Negeri Sunan Ampel Surabaya |
record_format | Article |
series | Biotropic: The Journal of Tropical Biology |
spelling | doaj.art-2d1596f73b684d49946ec3889f21e6892022-12-21T21:27:37ZengUniversitas Islam Negeri Sunan Ampel SurabayaBiotropic: The Journal of Tropical Biology2580-50292021-09-015210111510.29080/biotropic.2021.5.2.101-1151316Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel SurabayaFahimatul Ula0Misbakhul Munir1Hanik Faizah2Universitas Islam Negeri Sunan Ampel SurabayaUniversitas Islam Negeri Sunan Ampel SurabayaUniversitas Islam Negeri Sunan Ampel SurabayaThe condition of eating places such as street vendors will determine the level of food hygiene, including the water used to wash food utensils. This study aimed to determine the number of microbes and identify Escherichia coli and Salmonella sp. on the water that was used to wash tableware by street vendors around UIN Sunan Ampel Surabaya. Water samples before and after being used to wash tableware were obtained from 4 street vendors. The number of microbes in the sample was tested by the Total Plate Count (TPC) and Most Probable Number (MPN) methods, Escherichia coli was identified using Eosin Methylene Blue Agar (EMB) media, and Salmonella sp. was identified using Salmonella-Shigella agar (SSA) and Triple Sugar Iron agar (TSIA) media. Based on the results of TPC test, the water sample before being used to wash tableware had the lowest and highest values that were 3.0 x 102 Cfu/ml and 9.8 x 105 Cfu/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 4.0 x 102 Cfu/ml and 3.5 x 105 Cfu/ml, respectively. In the MPN test, the water sample before being used to wash tableware had the lowest and highest values that were 15/100ml and 26 100/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 72/100 ml and 271/ 100 ml, respectively. Escherichia coli was detected in all samples 1&3 while in sample 2 Escherichia coli was detected in the water before it was detected the genus Enterobacter and in samples 4 the genus Enterobacter was detected and all samples water used to wash tableware of street vendors was positive for Salmonella.http://jurnalsaintek.uinsby.ac.id/index.php/biotropic/article/view/1316tpccoliformmpnpedagang kaki limaair cucian peralatan makanan |
spellingShingle | Fahimatul Ula Misbakhul Munir Hanik Faizah Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya Biotropic: The Journal of Tropical Biology tpc coliform mpn pedagang kaki lima air cucian peralatan makanan |
title | Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya |
title_full | Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya |
title_fullStr | Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya |
title_full_unstemmed | Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya |
title_short | Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya |
title_sort | uji cemaran mikroba pada air yang digunakan untuk mencuci peralatan makan oleh pedagang kaki lima di sekitar uin sunan ampel surabaya |
topic | tpc coliform mpn pedagang kaki lima air cucian peralatan makanan |
url | http://jurnalsaintek.uinsby.ac.id/index.php/biotropic/article/view/1316 |
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