Glycerol Enhancement of Ligand-Polylysine/DNA Transfection
Primary human fibroblasts and a series of cell lines (A549, BNL CL.2, H225, NIH 3T3 and Rat-1) are efficiently transfected by using positively charged complexes of plasmid DNA and transferrin-polylysine or polylysine in the presence of glycerol (1 molar to 1.8 molar, depending on the cell type). An...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96205rr04 |