Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the Coronaviridae family which led to a global pandemic. However, available knowledge on adaptive immunity in convalescent individuals is limited. The present study was conducted on 191 convalescent coronavirus disease 2019 (COV...

Full description

Bibliographic Details
Main Authors: I Hasan Ali, A Sahib Abdulamir
Format: Article
Language:English
Published: Razi Vaccine and Serum Research Institute 2021-11-01
Series:Archives of Razi Institute
Subjects:
Online Access:https://archrazi.areeo.ac.ir/article_125026_bb4a929a1a5df4c59ccfeeb9f565e6f8.pdf
_version_ 1818245927669858304
author I Hasan Ali
A Sahib Abdulamir
author_facet I Hasan Ali
A Sahib Abdulamir
author_sort I Hasan Ali
collection DOAJ
description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the Coronaviridae family which led to a global pandemic. However, available knowledge on adaptive immunity in convalescent individuals is limited. The present study was conducted on 191 convalescent coronavirus disease 2019 (COVID-19) health care workers (HCW); moreover, it evaluated the cell-mediated immunity of 122 recovered HCW and the level of anti-receptor binding domain (RBD) IgG antibodies of 181 recovered HCW. Cellular and humoral immune responses were identified over time from one to eight months post recovery with varying disease severity using MTT proliferation assay and enzyme-linked immunosorbent assay. Analysis of lymphocyte proliferation with S1 protein in mild-moderate and severe HCW revealed an insignificant difference with an increase in the maximum and third quartile (Q3) from one to eight months after COVID-19 recovery. Antibody levels in mild-moderate and severe recovered HCW were insignificantly different from post-COVID 19 recovery (P>0.05); in addition, the median, maximum, and Q3 values of anti-RBD IgG were close to each other over the time intervals from one to eight months post recovery. These data suggest that many convalescent HCW enrolled in this study were re-exposed to the virus without the development of symptoms indicating the role of cell-mediated and humoral immunity in preventing symptomatic reinfection. This study reveals that a robust immunity developed after mild, moderate, and severe COVID-19 that could last for several months post recovery.
first_indexed 2024-12-12T14:40:42Z
format Article
id doaj.art-2d5c1dfba9b74c5b8f6e907a50910757
institution Directory Open Access Journal
issn 0365-3439
2008-9872
language English
last_indexed 2024-12-12T14:40:42Z
publishDate 2021-11-01
publisher Razi Vaccine and Serum Research Institute
record_format Article
series Archives of Razi Institute
spelling doaj.art-2d5c1dfba9b74c5b8f6e907a509107572022-12-22T00:21:14ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722021-11-017651255126710.22092/ari.2021.356135.1785125026Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in IraqI Hasan Ali0A Sahib Abdulamir1Department of Microbiology, Al-Nahrain University College of Medicine, Baghdad, IraqDepartment of Microbiology, Al-Nahrain University College of Medicine, Baghdad, IraqSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the Coronaviridae family which led to a global pandemic. However, available knowledge on adaptive immunity in convalescent individuals is limited. The present study was conducted on 191 convalescent coronavirus disease 2019 (COVID-19) health care workers (HCW); moreover, it evaluated the cell-mediated immunity of 122 recovered HCW and the level of anti-receptor binding domain (RBD) IgG antibodies of 181 recovered HCW. Cellular and humoral immune responses were identified over time from one to eight months post recovery with varying disease severity using MTT proliferation assay and enzyme-linked immunosorbent assay. Analysis of lymphocyte proliferation with S1 protein in mild-moderate and severe HCW revealed an insignificant difference with an increase in the maximum and third quartile (Q3) from one to eight months after COVID-19 recovery. Antibody levels in mild-moderate and severe recovered HCW were insignificantly different from post-COVID 19 recovery (P>0.05); in addition, the median, maximum, and Q3 values of anti-RBD IgG were close to each other over the time intervals from one to eight months post recovery. These data suggest that many convalescent HCW enrolled in this study were re-exposed to the virus without the development of symptoms indicating the role of cell-mediated and humoral immunity in preventing symptomatic reinfection. This study reveals that a robust immunity developed after mild, moderate, and severe COVID-19 that could last for several months post recovery.https://archrazi.areeo.ac.ir/article_125026_bb4a929a1a5df4c59ccfeeb9f565e6f8.pdfcell-mediated immunitycoronavirus disease 2019 (covid-19)humoral immunitysevere acute respiratory syndrome coronavirus 2 (sars-cov-2)
spellingShingle I Hasan Ali
A Sahib Abdulamir
Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq
Archives of Razi Institute
cell-mediated immunity
coronavirus disease 2019 (covid-19)
humoral immunity
severe acute respiratory syndrome coronavirus 2 (sars-cov-2)
title Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq
title_full Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq
title_fullStr Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq
title_full_unstemmed Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq
title_short Measurement of SARS-CoV-2-Specific Humoral and Cellular Immunity in Coronavirus Disease 2019 Convalescent Health Care Workers in Iraq
title_sort measurement of sars cov 2 specific humoral and cellular immunity in coronavirus disease 2019 convalescent health care workers in iraq
topic cell-mediated immunity
coronavirus disease 2019 (covid-19)
humoral immunity
severe acute respiratory syndrome coronavirus 2 (sars-cov-2)
url https://archrazi.areeo.ac.ir/article_125026_bb4a929a1a5df4c59ccfeeb9f565e6f8.pdf
work_keys_str_mv AT ihasanali measurementofsarscov2specifichumoralandcellularimmunityincoronavirusdisease2019convalescenthealthcareworkersiniraq
AT asahibabdulamir measurementofsarscov2specifichumoralandcellularimmunityincoronavirusdisease2019convalescenthealthcareworkersiniraq