ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress
Although RNA-Seq has revolutionized transcript analysis, organellar transcriptomes are rarely assessed even when present in published datasets. Here, we describe the development and application of a rapid and convenient method, ChloroSeq, to delineate qualitative and quantitative features of chlorop...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2016-09-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.116.030783 |
| _version_ | 1818999247282896896 |
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| author | Benoît Castandet Amber M. Hotto Susan R. Strickler David B. Stern |
| author_facet | Benoît Castandet Amber M. Hotto Susan R. Strickler David B. Stern |
| author_sort | Benoît Castandet |
| collection | DOAJ |
| description | Although RNA-Seq has revolutionized transcript analysis, organellar transcriptomes are rarely assessed even when present in published datasets. Here, we describe the development and application of a rapid and convenient method, ChloroSeq, to delineate qualitative and quantitative features of chloroplast RNA metabolism from strand-specific RNA-Seq datasets, including processing, editing, splicing, and relative transcript abundance. The use of a single experiment to analyze systematically chloroplast transcript maturation and abundance is of particular interest due to frequent pleiotropic effects observed in mutants that affect chloroplast gene expression and/or photosynthesis. To illustrate its utility, ChloroSeq was applied to published RNA-Seq datasets derived from Arabidopsis thaliana grown under control and abiotic stress conditions, where the organellar transcriptome had not been examined. The most appreciable effects were found for heat stress, which induces a global reduction in splicing and editing efficiency, and leads to increased abundance of chloroplast transcripts, including genic, intergenic, and antisense transcripts. Moreover, by concomitantly analyzing nuclear transcripts that encode chloroplast gene expression regulators from the same libraries, we demonstrate the possibility of achieving a holistic understanding of the nucleus-organelle system. ChloroSeq thus represents a unique method for streamlining RNA-Seq data interpretation of the chloroplast transcriptome and its regulators. |
| first_indexed | 2024-12-20T22:14:23Z |
| format | Article |
| id | doaj.art-2d85983c1f0e4ae0b1da8cff1d2781b5 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-20T22:14:23Z |
| publishDate | 2016-09-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-2d85983c1f0e4ae0b1da8cff1d2781b52022-12-21T19:25:05ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362016-09-01692817282710.1534/g3.116.03078314ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat StressBenoît CastandetAmber M. HottoSusan R. StricklerDavid B. SternAlthough RNA-Seq has revolutionized transcript analysis, organellar transcriptomes are rarely assessed even when present in published datasets. Here, we describe the development and application of a rapid and convenient method, ChloroSeq, to delineate qualitative and quantitative features of chloroplast RNA metabolism from strand-specific RNA-Seq datasets, including processing, editing, splicing, and relative transcript abundance. The use of a single experiment to analyze systematically chloroplast transcript maturation and abundance is of particular interest due to frequent pleiotropic effects observed in mutants that affect chloroplast gene expression and/or photosynthesis. To illustrate its utility, ChloroSeq was applied to published RNA-Seq datasets derived from Arabidopsis thaliana grown under control and abiotic stress conditions, where the organellar transcriptome had not been examined. The most appreciable effects were found for heat stress, which induces a global reduction in splicing and editing efficiency, and leads to increased abundance of chloroplast transcripts, including genic, intergenic, and antisense transcripts. Moreover, by concomitantly analyzing nuclear transcripts that encode chloroplast gene expression regulators from the same libraries, we demonstrate the possibility of achieving a holistic understanding of the nucleus-organelle system. ChloroSeq thus represents a unique method for streamlining RNA-Seq data interpretation of the chloroplast transcriptome and its regulators.http://g3journal.org/lookup/doi/10.1534/g3.116.030783Arabidopsis thalianachloroplastRNA-Seqheat stresstranscriptomenoncoding RNAsintronsRNA splicingRNA editing |
| spellingShingle | Benoît Castandet Amber M. Hotto Susan R. Strickler David B. Stern ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress G3: Genes, Genomes, Genetics Arabidopsis thaliana chloroplast RNA-Seq heat stress transcriptome noncoding RNAs introns RNA splicing RNA editing |
| title | ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress |
| title_full | ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress |
| title_fullStr | ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress |
| title_full_unstemmed | ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress |
| title_short | ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress |
| title_sort | chloroseq an optimized chloroplast rna seq bioinformatic pipeline reveals remodeling of the organellar transcriptome under heat stress |
| topic | Arabidopsis thaliana chloroplast RNA-Seq heat stress transcriptome noncoding RNAs introns RNA splicing RNA editing |
| url | http://g3journal.org/lookup/doi/10.1534/g3.116.030783 |
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