A protocol for isolation and culturing of mouse primary postmitotic photoreceptors and isolation of extracellular vesicles

A protocol for isolation and culturing of mouse primary postmitotic photoreceptors and isolation of extracellular vesicles

Summary: Here, we present a protocol for isolating and culturing mouse photoreceptors in a minimal, chemically defined medium free from serum. We describe steps for retina dissection, enzymatic dissociation, photoreceptor enrichment, cell culture, extracellular vesicles (EVs) enrichment, and EV ultr...

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Bibliographic Details
Main Authors: Aikaterini A. Kalargyrou, Ayako Matsuyama, Emily P. Lanning, Mahmoud Khazim, Siobhan Guilfoyle, Alexander J. Smith, Robin R. Ali, Rachael A. Pearson
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Molecular Biology
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000406

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http://www.sciencedirect.com/science/article/pii/S2666166724000406

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