Reengineering of 7-dehydrocholesterol biosynthesis in Saccharomyces cerevisiae using combined pathway and organelle strategies

Reengineering of 7-dehydrocholesterol biosynthesis in Saccharomyces cerevisiae using combined pathway and organelle strategies

7-Dehydrocholesterol (7-DHC) is a widely used sterol and a precursor of several costly steroidal drugs. In this study, 7-DHC biosynthesis pathway was constructed and modified in Saccharomyces cerevisiae. Firstly, the biosynthesis pathway was constructed by knocking out the competitive pathway genes...

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Bibliographic Details
Main Authors: Wenqian Wei, Song Gao, Qiong Yi, Anjian Liu, Shiqin Yu, Jingwen Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Microbiology
Subjects:
7-dehydrocholesterol
“push and pull”
multi-copy site integration
metabolic engineering
Saccharomyces cerevisiae
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.978074/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.978074/full

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