Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogens
Abstract Antibiotic resistance or tolerance of pathogens is one of the most serious global public health threats. Bacteria in biofilms show extreme tolerance to almost all antibiotic classes. Thus, use of antibiofilm drugs without bacterial‐killing effects is one of the strategies to combat antibiot...
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | mLife |
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| Online Access: | https://doi.org/10.1002/mlf2.12087 |
| _version_ | 1797671724324487168 |
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| author | Yu Zhang Pramod Bhasme Dinesh S. Reddy Dejian Liu Zhaoxiao Yu Tianhu Zhao Yaqian Zheng Amit Kumar Haiying Yu Luyan Z. Ma |
| author_facet | Yu Zhang Pramod Bhasme Dinesh S. Reddy Dejian Liu Zhaoxiao Yu Tianhu Zhao Yaqian Zheng Amit Kumar Haiying Yu Luyan Z. Ma |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | Abstract Antibiotic resistance or tolerance of pathogens is one of the most serious global public health threats. Bacteria in biofilms show extreme tolerance to almost all antibiotic classes. Thus, use of antibiofilm drugs without bacterial‐killing effects is one of the strategies to combat antibiotic tolerance. In this study, we discovered a coumarin–chalcone conjugate C9, which can inhibit the biofilm formation of three common pathogens that cause nosocomial infections, namely, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, with the best antibiofilm activity against P. aeruginosa. Further investigations indicate that C9 decreases the synthesis of the key biofilm matrix exopolysaccharide Psl and bacterial second messenger cyclic‐di‐GMP. Meanwhile, C9 can interfere with the regulation of the quorum sensing (QS) system to reduce the virulence of P. aeruginosa. C9 treatment enhances the sensitivity of biofilm to several antibiotics and reduces the survival rate of P. aeruginosa under starvation or oxidative stress conditions, indicating its excellent potential for use as an antibiofilm‐forming and anti‐QS drug. |
| first_indexed | 2024-03-11T21:19:42Z |
| format | Article |
| id | doaj.art-2dd84a9cf003493eb4e73a2b7d8cc36c |
| institution | Directory Open Access Journal |
| issn | 2770-100X |
| language | English |
| last_indexed | 2024-03-11T21:19:42Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | mLife |
| spelling | doaj.art-2dd84a9cf003493eb4e73a2b7d8cc36c2023-09-28T10:25:52ZengWileymLife2770-100X2023-09-012328329410.1002/mlf2.12087Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogensYu Zhang0Pramod Bhasme1Dinesh S. Reddy2Dejian Liu3Zhaoxiao Yu4Tianhu Zhao5Yaqian Zheng6Amit Kumar7Haiying Yu8Luyan Z. Ma9State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaCentre for Nano and Material Sciences Jain University Bangalore Karnataka IndiaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaCentre for Nano and Material Sciences Jain University Bangalore Karnataka IndiaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing ChinaAbstract Antibiotic resistance or tolerance of pathogens is one of the most serious global public health threats. Bacteria in biofilms show extreme tolerance to almost all antibiotic classes. Thus, use of antibiofilm drugs without bacterial‐killing effects is one of the strategies to combat antibiotic tolerance. In this study, we discovered a coumarin–chalcone conjugate C9, which can inhibit the biofilm formation of three common pathogens that cause nosocomial infections, namely, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, with the best antibiofilm activity against P. aeruginosa. Further investigations indicate that C9 decreases the synthesis of the key biofilm matrix exopolysaccharide Psl and bacterial second messenger cyclic‐di‐GMP. Meanwhile, C9 can interfere with the regulation of the quorum sensing (QS) system to reduce the virulence of P. aeruginosa. C9 treatment enhances the sensitivity of biofilm to several antibiotics and reduces the survival rate of P. aeruginosa under starvation or oxidative stress conditions, indicating its excellent potential for use as an antibiofilm‐forming and anti‐QS drug.https://doi.org/10.1002/mlf2.12087biofilmcyclic‐di‐GMPexopolysaccharide PslPseudomonas aeruginosaquorum sensing |
| spellingShingle | Yu Zhang Pramod Bhasme Dinesh S. Reddy Dejian Liu Zhaoxiao Yu Tianhu Zhao Yaqian Zheng Amit Kumar Haiying Yu Luyan Z. Ma Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogens mLife biofilm cyclic‐di‐GMP exopolysaccharide Psl Pseudomonas aeruginosa quorum sensing |
| title | Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogens |
| title_full | Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogens |
| title_fullStr | Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogens |
| title_full_unstemmed | Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogens |
| title_short | Dual functions: A coumarin–chalcone conjugate inhibits cyclic‐di‐GMP and quorum‐sensing signaling to reduce biofilm formation and virulence of pathogens |
| title_sort | dual functions a coumarin chalcone conjugate inhibits cyclic di gmp and quorum sensing signaling to reduce biofilm formation and virulence of pathogens |
| topic | biofilm cyclic‐di‐GMP exopolysaccharide Psl Pseudomonas aeruginosa quorum sensing |
| url | https://doi.org/10.1002/mlf2.12087 |
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