Predictable NHEJ Insertion and Assessment of HDR Editing Strategies in Plants

Canonical CRISPR-Cas9 genome editing technique has profoundly impacted the fields of plant biology, biotechnology, and crop improvement. Since non-homologous end joining (NHEJ) is usually considered to generate random indels, its high efficiency mutation is generally not pertinent to precise editing...

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Bibliographic Details
Main Authors: Kutubuddin A. Molla, Justin Shih, Matthew S. Wheatley, Yinong Yang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-03-01
Series:Frontiers in Genome Editing
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2022.825236/full