Polyethylene glycol and polyvinylpirrolidone effect on bacterial rRNA extraction and hybridization from cells exposed to tannins Efeito de polietilenoglicol e polivinilpirrolidona na extração e hibridização de rRNA bacteriano de células expostas a taninos

Polyethylene glycol and polyvinylpirrolidone effect on bacterial rRNA extraction and hybridization from cells exposed to tannins Efeito de polietilenoglicol e polivinilpirrolidona na extração e hibridização de rRNA bacteriano de células expostas a taninos

In order to detect fluctuations in ruminal microbial populations due to forage tannins using 16S ribosomal RNA (rRNA) probes, recovery of intact rRNA is required. The objective of this work was to evaluate the effect of polyethylene glycol (PEG) and polyvinylpirrolidone (PVP) on extraction of bacter...

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Bibliographic Details
Main Authors: Pedro Braga Arcuri, Michael Larry Thonney, Peter Schofield, Alice Nelson Pell
Format: Article
Language:English
Published: Embrapa Informação Tecnológica 2003-09-01
Series:Pesquisa Agropecuária Brasileira
Subjects:
Ruminococcus albus
ecologia microbiana
nutrição animal
microbial ecology
animal nutrition
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000900008

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000900008

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