Protocol for CRISPR-Cas9-mediated genome editing to study spermatogenesis in Caenorhabditis elegans

Protocol for CRISPR-Cas9-mediated genome editing to study spermatogenesis in Caenorhabditis elegans

Summary: Gene silencing by P-element-induced wimpy testis-interacting RNAs is a mechanism to maintain genome integrity in germ cells. Here, we present a protocol for knockin or knockout editing of male germline genome mediated by CRISPR-Cas9 technology in Caenorhabditis elegans. We describe steps fo...

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Bibliographic Details
Main Authors: Peng Wang, Zheng Cao, Qiushi Wang, Xiaojing Ma, Ning Wang, Lianwan Chen, Yanmei Zhao, Long Miao
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Developmental biology
Genetics
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006871

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723006871

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