Protocol for CRISPR-Cas9-mediated genome editing to study spermatogenesis in Caenorhabditis elegans
Summary: Gene silencing by P-element-induced wimpy testis-interacting RNAs is a mechanism to maintain genome integrity in germ cells. Here, we present a protocol for knockin or knockout editing of male germline genome mediated by CRISPR-Cas9 technology in Caenorhabditis elegans. We describe steps fo...
Main Authors: | Peng Wang, Zheng Cao, Qiushi Wang, Xiaojing Ma, Ning Wang, Lianwan Chen, Yanmei Zhao, Long Miao |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-12-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723006871 |
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