Application of FT near spectroscopy for determination of true protein and casein in milk

Our study deals with a possibility of determining true protein and casein in cow's, ewe's and goat's milk and in ewe's colostrums by FT NIR spectroscopy. Samples of milk were analysed by FT NIR in the reflectance mode with the transflectance cuvette. The values of correlation coe...

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Main Authors: K. Šustová, J. Růžičková, J. Kuchtík
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2007-09-01
Series:Czech Journal of Animal Science
Subjects:
Online Access:https://cjas.agriculturejournals.cz/artkey/cjs-200709-0003_application-of-ft-near-spectroscopy-for-determination-of-true-protein-and-casein-in-milk.php
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author K. Šustová
J. Růžičková
J. Kuchtík
author_facet K. Šustová
J. Růžičková
J. Kuchtík
author_sort K. Šustová
collection DOAJ
description Our study deals with a possibility of determining true protein and casein in cow's, ewe's and goat's milk and in ewe's colostrums by FT NIR spectroscopy. Samples of milk were analysed by FT NIR in the reflectance mode with the transflectance cuvette. The values of correlation coefficients of calibration were as follows: cow's protein 0.943; cow's casein 0.964; ewe's protein 0.997; ewe's casein 0.977; goat's protein 0.989; goat's casein 0.890; ewe's colostrum protein 0.983. Calibration was tested using the same set of samples by the cross validation method. The values of correlation coefficients of validation were as follows: cow's protein 0.923; cow's casein 0.910; ewe's protein 0.994; ewe's casein 0.963; goat's protein 0.972; goat's casein 0.814; ewe's colostrum protein 0.871. The NIRS results were compared with reference data and no significant differences between them were found (P = 0.05). Results of this study indicate that FT NIR spectroscopy can be used for a rapid analysis of protein and casein in cow's, ewe's and goat's milk and ewe's colostrum.
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spelling doaj.art-2e4b26e111c94775a018c73f8731da912023-02-23T03:32:29ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092007-09-0152928429110.17221/2264-CJAScjs-200709-0003Application of FT near spectroscopy for determination of true protein and casein in milkK. Šustová0J. Růžičková1J. Kuchtík2Department of Food Technology, Mendel University of Agriculture and Forestry in Brno, Czech RepublicDepartment of Food Technology, Mendel University of Agriculture and Forestry in Brno, Czech RepublicDepartment of Animal Breeding, Mendel University of Agriculture and Forestry in Brno, Czech RepublicOur study deals with a possibility of determining true protein and casein in cow's, ewe's and goat's milk and in ewe's colostrums by FT NIR spectroscopy. Samples of milk were analysed by FT NIR in the reflectance mode with the transflectance cuvette. The values of correlation coefficients of calibration were as follows: cow's protein 0.943; cow's casein 0.964; ewe's protein 0.997; ewe's casein 0.977; goat's protein 0.989; goat's casein 0.890; ewe's colostrum protein 0.983. Calibration was tested using the same set of samples by the cross validation method. The values of correlation coefficients of validation were as follows: cow's protein 0.923; cow's casein 0.910; ewe's protein 0.994; ewe's casein 0.963; goat's protein 0.972; goat's casein 0.814; ewe's colostrum protein 0.871. The NIRS results were compared with reference data and no significant differences between them were found (P = 0.05). Results of this study indicate that FT NIR spectroscopy can be used for a rapid analysis of protein and casein in cow's, ewe's and goat's milk and ewe's colostrum.https://cjas.agriculturejournals.cz/artkey/cjs-200709-0003_application-of-ft-near-spectroscopy-for-determination-of-true-protein-and-casein-in-milk.phpnear-infrared spectroscopycow's milkewe's milkgoat's milkcolostrumtrue proteincaseinchemical composition
spellingShingle K. Šustová
J. Růžičková
J. Kuchtík
Application of FT near spectroscopy for determination of true protein and casein in milk
Czech Journal of Animal Science
near-infrared spectroscopy
cow's milk
ewe's milk
goat's milk
colostrum
true protein
casein
chemical composition
title Application of FT near spectroscopy for determination of true protein and casein in milk
title_full Application of FT near spectroscopy for determination of true protein and casein in milk
title_fullStr Application of FT near spectroscopy for determination of true protein and casein in milk
title_full_unstemmed Application of FT near spectroscopy for determination of true protein and casein in milk
title_short Application of FT near spectroscopy for determination of true protein and casein in milk
title_sort application of ft near spectroscopy for determination of true protein and casein in milk
topic near-infrared spectroscopy
cow's milk
ewe's milk
goat's milk
colostrum
true protein
casein
chemical composition
url https://cjas.agriculturejournals.cz/artkey/cjs-200709-0003_application-of-ft-near-spectroscopy-for-determination-of-true-protein-and-casein-in-milk.php
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AT jkuchtik applicationofftnearspectroscopyfordeterminationoftrueproteinandcaseininmilk