Improved protocol for single-nucleus RNA-sequencing of frozen human bladder tumor biopsies

Improved protocol for single-nucleus RNA-sequencing of frozen human bladder tumor biopsies

ABSTRACTThis paper provides a laboratory workflow for single-nucleus RNA-sequencing (snRNA-seq) including a protocol for gentle nuclei isolation from fresh frozen tumor biopsies, making it possible to analyze biobanked material. To develop this protocol, we used non-frozen and frozen human bladder t...

Full description

Bibliographic Details
Main Authors: Sofie S. Schmøkel, Iver Nordentoft, Sia V. Lindskrog, Philippe Lamy, Michael Knudsen, Jørgen Bjerggaard Jensen, Lars Dyrskjøt
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Nucleus
Subjects:
10× chromium
bladder cancer
Drop-seq
DroNc-seq
nuclei isolation
single-cell analysis
Online Access:https://www.tandfonline.com/doi/10.1080/19491034.2023.2186686

Internet

https://www.tandfonline.com/doi/10.1080/19491034.2023.2186686

Similar Items