PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2′-deoxycytidine 5′-triphosphate

PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2′-deoxycytidine 5′-triphosphate

GC-rich DNA regions were PCR-amplified with Taq DNA polymerase using either the canonical set of deoxynucleoside triphosphates or mixtures in which the dCTP had been partially or completely replaced by its N4-methylated analog, N4-methyl-2′-deoxycytidine 5′-triphosphate (N4me-dCTP). In the case of a...

Szczegółowa specyfikacja

Opis bibliograficzny
Główni autorzy: Cyntia R. Flores-Juárez, Eva González-Jasso, Anaid Antaramian, Reynaldo C. Pless
Format: Artykuł
Język:English
Wydane: Taylor & Francis Group 2016-10-01
Seria:BioTechniques
Hasła przedmiotowe:
GC-rich DNA
PCR
N4-methylcytosine
dCTP analog
Dostęp online:https://www.future-science.com/doi/10.2144/000114457

Internet

https://www.future-science.com/doi/10.2144/000114457

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