Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions

Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions

Abstract Production of recombinant proteins such as antibody fragments in the periplasm of the bacterium Escherichia coli has a number of advantages, including the ability to form disulphide bonds, aiding correct folding, and the relative ease of release and subsequent capture and purification. In t...

Full description

Bibliographic Details
Main Authors: Ikhlaas M. Kasli, Owen R. T. Thomas, Tim W. Overton
Format: Article
Language:English
Published: SpringerOpen 2019-01-01
Series:AMB Express
Subjects:
Fermentation
Heterologous protein
Biopharmaceutical
Single-chain variable fragment (scFv)
Online Access:http://link.springer.com/article/10.1186/s13568-018-0727-8

Internet

http://link.springer.com/article/10.1186/s13568-018-0727-8

Similar Items