Establishment of loop-mediated isothermal amplification for Brucella detection using a warmer pad as a heating source
The study sought to establish a sensitive and specific on-site loop-mediated isothermal amplification (LAMP) for Brucella heated using a warmer pad. LAMP primers specific to the conserved BvrR gene were designed, and the LAMP reaction was optimized. The heating characteristics of the warmer pad were...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2022-09-01
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Series: | BioTechniques |
Subjects: | |
Online Access: | https://www.future-science.com/doi/10.2144/btn-2021-0043 |