Establishment of loop-mediated isothermal amplification for Brucella detection using a warmer pad as a heating source

The study sought to establish a sensitive and specific on-site loop-mediated isothermal amplification (LAMP) for Brucella heated using a warmer pad. LAMP primers specific to the conserved BvrR gene were designed, and the LAMP reaction was optimized. The heating characteristics of the warmer pad were...

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Bibliographic Details
Main Authors: Ye Yin-Bo, Li Dong-Liang, Yang Bo-Han, Zhang Zhao, Mei Si-Yao, Liu Bao-Shan, Chen Ze-Liang
Format: Article
Language:English
Published: Taylor & Francis Group 2022-09-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2021-0043