Genome-wide Specificity of Highly Efficient TALENs and CRISPR/Cas9 for T Cell Receptor Modification

Genome-wide Specificity of Highly Efficient TALENs and CRISPR/Cas9 for T Cell Receptor Modification

In T cells with transgenic high-avidity T cell receptors (TCRs), endogenous and transferred TCR chains compete for surface expression and may pair inappropriately, potentially causing autoimmunity. To knock out endogenous TCR expression, we assembled 12 transcription activator-like effector nuclease...

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Bibliographic Details
Main Authors: Friederike Knipping, Mark J. Osborn, Karl Petri, Jakub Tolar, Hanno Glimm, Christof von Kalle, Manfred Schmidt, Richard Gabriel
Format: Article
Language:English
Published: Elsevier 2017-03-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
gene editing
T cell therapy
off target
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050117300098

Internet

http://www.sciencedirect.com/science/article/pii/S2329050117300098

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