Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators

Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators

Summary: Ferroptosis, an iron-dependent programmed cell death triggered by excessive lipid peroxidation, has shown promising therapeutic potentials in human diseases. Here, we describe a protocol of a CRISPR-Cas9 loss-of-function screen to identify regulators in response to different inducers of fer...

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Bibliographic Details
Main Authors: Xin Yang, Shoufu Duan, Zhiming Li, Zhe Wang, Ning Kon, Zhiguo Zhang, Wei Gu
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Cell Biology
Cell culture
Cell-based Assays
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007293

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http://www.sciencedirect.com/science/article/pii/S2666166723007293

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