Phosphoinositide detection at synapses of fixed murine hippocampal neurons

Phosphoinositide detection at synapses of fixed murine hippocampal neurons

Summary: The minor phospholipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is crucial for neurotransmission and has been implicated in Parkinson’s disease. Here, we present a staining protocol for the analysis of activity-dependent changes of PI(4,5)P2 at synapses. We describe steps for stimu...

Full description

Bibliographic Details
Main Authors: Svenja Bolz, Natalie Kaempf, Maria Muehlbauer, Delia Löwe, Volker Haucke
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Membrane
Microscopy
Antibody
Neuroscience
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001102

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724001102

Similar Items