Phosphoinositide detection at synapses of fixed murine hippocampal neurons

Phosphoinositide detection at synapses of fixed murine hippocampal neurons

Summary: The minor phospholipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is crucial for neurotransmission and has been implicated in Parkinson’s disease. Here, we present a staining protocol for the analysis of activity-dependent changes of PI(4,5)P2 at synapses. We describe steps for stimu...

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Bibliographic Details
Main Authors:	Svenja Bolz, Natalie Kaempf, Maria Muehlbauer, Delia Löwe, Volker Haucke
Format:	Article
Language:	English
Published:	Elsevier 2024-06-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell Membrane Microscopy Antibody Neuroscience Protein expression and purification
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724001102

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