Minimal genome-wide human CRISPR-Cas9 library
Abstract CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-f...
Main Authors: | , , , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2021-01-01
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Series: | Genome Biology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13059-021-02268-4 |