Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation

Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation

CRISPR/Cas9 protein fused to transactivation domains can be used to control gene expression in human cells. In this study, we demonstrate that a dCas9 fusion with repeats of VP16 activator domains can efficiently activate human genes involved in pluripotency in various cell types. This activator in...

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Bibliographic Details
Main Authors: Diego Balboa, Jere Weltner, Solja Eurola, Ras Trokovic, Kirmo Wartiovaara, Timo Otonkoski
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671115002234

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http://www.sciencedirect.com/science/article/pii/S2213671115002234

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