Cis-Element Engineering Promotes the Expression of <i>Bacillus subtilis</i> Type I L-Asparaginase and Its Application in Food

Cis-Element Engineering Promotes the Expression of <i>Bacillus subtilis</i> Type I L-Asparaginase and Its Application in Food

Type I L-asparaginase from <i>Bacillus licheniformis</i> Z-1 (BlAase) was efficiently produced and secreted in <i>Bacillus subtilis</i> RIK 1285, but its low yield made it unsuitable for industrial use. Thus, a combined method was used in this study to boost BlAase synthesis...

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Bibliographic Details
Main Authors: Jiafeng Niu, Ruxue Yan, Juan Shen, Xiaoyu Zhu, Fanqiang Meng, Zhaoxin Lu, Fengxia Lu
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:International Journal of Molecular Sciences
Subjects:
L-asparaginase
<i>Bacillus subtilis</i>
promoter
dual-promoter systems
ribosome binding site
scale-up fermentation
Online Access:https://www.mdpi.com/1422-0067/23/12/6588

Internet

https://www.mdpi.com/1422-0067/23/12/6588

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