Development and application of a fast and efficient CRISPR-based genetic toolkit in Bacillus amyloliquefaciens LB1ba02

Abstract Background Bacillus amyloliquefaciens is generally recognized as food safe (GRAS) microbial host and important enzyme-producing strain in the industry. B.amyloliquefaciens LB1ba02 is a production strain suitable for secreting mesophilic α-amylase in the industry. Nevertheless, due to the lo...

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Bibliographic Details
Main Authors: Qinglong Xin, Yudan Chen, Qianlin Chen, Bin Wang, Li Pan
Format: Article
Language:English
Published: BMC 2022-05-01
Series:Microbial Cell Factories
Subjects:
Online Access:https://doi.org/10.1186/s12934-022-01832-2