Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movement

Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movement

In this article, we provide detailed protocols on using optogenetic dimerizers to acutely perturb activities of guanine nucleotide exchange factors (GEFs) specific to Ras, Rac or Rho small GTPases of the migratory networks in various mammalian and amoeba cell lines. These GEFs are crucial components...

Full description

Bibliographic Details
Main Authors: Dhiman Sankar Pal, Yiyan Lin, Huiwang Zhan, Tatsat Banerjee, Jonathan Kuhn, Stephenie Providence, Peter N. Devreotes
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-07-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
optogenetics
development
growth factor signaling
actin cytoskeleton
immunity
cancer metastasis
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2023.1195806/full

Internet

https://www.frontiersin.org/articles/10.3389/fcell.2023.1195806/full

Similar Items