Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep
A critical hindrance in the development of effective vaccine strategies to combat infectious disease is lack of knowledge about correlates of protection and of the host responses necessary for successful adaptive immunity. Often vaccine formulations are developed by stepwise experimentation, with in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1004237/full |
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| author | Auriol C. Purdie Karren M. Plain Hannah Pooley Douglas J. Begg Kumudika de Silva Richard J. Whittington |
| author_facet | Auriol C. Purdie Karren M. Plain Hannah Pooley Douglas J. Begg Kumudika de Silva Richard J. Whittington |
| author_sort | Auriol C. Purdie |
| collection | DOAJ |
| description | A critical hindrance in the development of effective vaccine strategies to combat infectious disease is lack of knowledge about correlates of protection and of the host responses necessary for successful adaptive immunity. Often vaccine formulations are developed by stepwise experimentation, with incomplete investigation of the fundamental mechanisms of protection. Gudair® is a commercially available vaccine registered for use in sheep and goats for controlling spread of Mycobacterium avium sub-species paratuberculosis (MAP) infections and reduces mortality by up to 90%. Here, using an experimental infection model in sheep, we have utilized a transcriptomics approach to identify white blood cell gene expression changes in vaccinated, MAP-exposed Merino sheep with a protective response in comparison to those vaccinated animals that failed to develop immunity to MAP infection. This methodology facilitated an overview of gene-associated functional pathway adaptations using an in-silico analysis approach. We identified a group of genes that were activated in the vaccine-protected animals and confirmed stability of expression in samples obtained from naturally exposed commercially maintained sheep. We propose these genes as correlates of vaccine induced protection. |
| first_indexed | 2024-04-11T06:39:03Z |
| format | Article |
| id | doaj.art-2fefba701a5542f19c9f7b0c2feca63b |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T06:39:03Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-2fefba701a5542f19c9f7b0c2feca63b2022-12-22T04:39:35ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-11-01910.3389/fvets.2022.10042371004237Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheepAuriol C. PurdieKarren M. PlainHannah PooleyDouglas J. BeggKumudika de SilvaRichard J. WhittingtonA critical hindrance in the development of effective vaccine strategies to combat infectious disease is lack of knowledge about correlates of protection and of the host responses necessary for successful adaptive immunity. Often vaccine formulations are developed by stepwise experimentation, with incomplete investigation of the fundamental mechanisms of protection. Gudair® is a commercially available vaccine registered for use in sheep and goats for controlling spread of Mycobacterium avium sub-species paratuberculosis (MAP) infections and reduces mortality by up to 90%. Here, using an experimental infection model in sheep, we have utilized a transcriptomics approach to identify white blood cell gene expression changes in vaccinated, MAP-exposed Merino sheep with a protective response in comparison to those vaccinated animals that failed to develop immunity to MAP infection. This methodology facilitated an overview of gene-associated functional pathway adaptations using an in-silico analysis approach. We identified a group of genes that were activated in the vaccine-protected animals and confirmed stability of expression in samples obtained from naturally exposed commercially maintained sheep. We propose these genes as correlates of vaccine induced protection.https://www.frontiersin.org/articles/10.3389/fvets.2022.1004237/fullJohne's diseaseMycobacterium avium subsp. paratuberculosissheeptranscriptomicsGudair® vaccinecorrelates of protection |
| spellingShingle | Auriol C. Purdie Karren M. Plain Hannah Pooley Douglas J. Begg Kumudika de Silva Richard J. Whittington Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep Frontiers in Veterinary Science Johne's disease Mycobacterium avium subsp. paratuberculosis sheep transcriptomics Gudair® vaccine correlates of protection |
| title | Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep |
| title_full | Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep |
| title_fullStr | Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep |
| title_full_unstemmed | Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep |
| title_short | Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep |
| title_sort | correlates of vaccine protection against mycobacterium avium sub species paratuberculosis infection revealed in a transcriptomic study of responses in gudair r vaccinated sheep |
| topic | Johne's disease Mycobacterium avium subsp. paratuberculosis sheep transcriptomics Gudair® vaccine correlates of protection |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1004237/full |
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