Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
Abstract A novel liver-targeting interferon (IFN-CSP) was successfully over-expressed in our previous work. The in vitro and in vivo investigation revealed that IFN-CSP has significant anti-hepatitis B virus (HBV) effect and liver-targeting capacity. However, due to the IFN-CSP tends to form inclusi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-10-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0493-z |
| _version_ | 1828309305509543936 |
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| author | Along Liu Shuiqing Gui Lun Zhang Zhaoxia Chen Yanan Tang Mingzhu Xiao Jie Wang Wenbin Liu Xiaobao Jin Jiayong Zhu Xuemei Lu |
| author_facet | Along Liu Shuiqing Gui Lun Zhang Zhaoxia Chen Yanan Tang Mingzhu Xiao Jie Wang Wenbin Liu Xiaobao Jin Jiayong Zhu Xuemei Lu |
| author_sort | Along Liu |
| collection | DOAJ |
| description | Abstract A novel liver-targeting interferon (IFN-CSP) was successfully over-expressed in our previous work. The in vitro and in vivo investigation revealed that IFN-CSP has significant anti-hepatitis B virus (HBV) effect and liver-targeting capacity. However, due to the IFN-CSP tends to form inclusion bodies in recombinant Escherichia coli (E. coli), efficient production of the soluble liver-targeting interferon is a challenge. In view of biomedical application, novel strategies for efficiently expressing liver-targeting interferon and overcoming its poor solubility are necessary and important. In the present study, a modified mu-IFN-CSP was designed base on the amino acid mutant of the native IFN-CSP. Meanwhile, the coding sequence of mu-IFN-CSP was optimized for E. coli preferred codon and the induction conditions for expression were optimized by an orthogonal test. After amino acid mutant, codon optimization and induction conditions optimization, the solubility of Mu-IFN-CSP in E. coli was up to 98.4%. The structural comparison and molecular dynamic simulation showed that the Mu-IFN-CSP formed three structure changes and were more stable than the native IFN-CSP. Tissue sections binding assays revealed that Mu-IFN-CSP was also able to specific binding to liver. In vitro anti-HBV activity assays showed that the soluble Mu-IFN-CSP has improved anti-HBV effect in HepG2.2.15 cells compared to the native IFN-CSP. The present study reports for the first time that liver-targeting interferon Mu-IFN-CSP can be expressed as soluble form, and also contributes to further support its application as liver-targeting anti-HBV medicine. |
| first_indexed | 2024-04-13T15:26:27Z |
| format | Article |
| id | doaj.art-301a24ec1d864af6997be6784f59e4f4 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-04-13T15:26:27Z |
| publishDate | 2017-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-301a24ec1d864af6997be6784f59e4f42022-12-22T02:41:29ZengSpringerOpenAMB Express2191-08552017-10-017111310.1186/s13568-017-0493-zProduction of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expressionAlong Liu0Shuiqing Gui1Lun Zhang2Zhaoxia Chen3Yanan Tang4Mingzhu Xiao5Jie Wang6Wenbin Liu7Xiaobao Jin8Jiayong Zhu9Xuemei Lu10School of Basic Courses, Guangdong Pharmaceutical UniversityIntensive Care Unit, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversitySchool of Basic Courses, Guangdong Pharmaceutical UniversityAbstract A novel liver-targeting interferon (IFN-CSP) was successfully over-expressed in our previous work. The in vitro and in vivo investigation revealed that IFN-CSP has significant anti-hepatitis B virus (HBV) effect and liver-targeting capacity. However, due to the IFN-CSP tends to form inclusion bodies in recombinant Escherichia coli (E. coli), efficient production of the soluble liver-targeting interferon is a challenge. In view of biomedical application, novel strategies for efficiently expressing liver-targeting interferon and overcoming its poor solubility are necessary and important. In the present study, a modified mu-IFN-CSP was designed base on the amino acid mutant of the native IFN-CSP. Meanwhile, the coding sequence of mu-IFN-CSP was optimized for E. coli preferred codon and the induction conditions for expression were optimized by an orthogonal test. After amino acid mutant, codon optimization and induction conditions optimization, the solubility of Mu-IFN-CSP in E. coli was up to 98.4%. The structural comparison and molecular dynamic simulation showed that the Mu-IFN-CSP formed three structure changes and were more stable than the native IFN-CSP. Tissue sections binding assays revealed that Mu-IFN-CSP was also able to specific binding to liver. In vitro anti-HBV activity assays showed that the soluble Mu-IFN-CSP has improved anti-HBV effect in HepG2.2.15 cells compared to the native IFN-CSP. The present study reports for the first time that liver-targeting interferon Mu-IFN-CSP can be expressed as soluble form, and also contributes to further support its application as liver-targeting anti-HBV medicine.http://link.springer.com/article/10.1186/s13568-017-0493-zLiver-targeting interferonAmino acid mutantPreferred codon optimizedEscherichia coliSoluble expressionExpression conditions optimized |
| spellingShingle | Along Liu Shuiqing Gui Lun Zhang Zhaoxia Chen Yanan Tang Mingzhu Xiao Jie Wang Wenbin Liu Xiaobao Jin Jiayong Zhu Xuemei Lu Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression AMB Express Liver-targeting interferon Amino acid mutant Preferred codon optimized Escherichia coli Soluble expression Expression conditions optimized |
| title | Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression |
| title_full | Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression |
| title_fullStr | Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression |
| title_full_unstemmed | Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression |
| title_short | Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression |
| title_sort | production of bioactive liver targeting interferon mu ifn csp by soluble prokaryotic expression |
| topic | Liver-targeting interferon Amino acid mutant Preferred codon optimized Escherichia coli Soluble expression Expression conditions optimized |
| url | http://link.springer.com/article/10.1186/s13568-017-0493-z |
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