A murine model of elastase- and cigarette smoke-induced emphysema
ABSTRACT Objective: To describe a murine model of emphysema induced by a combination of exposure to cigarette smoke (CS) and instillation of porcine pancreatic elastase (PPE). Methods: A total of 38 C57BL/6 mice were randomly divided into four groups: control (one intranasal instillation of 0.9% s...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pneumologia e Tisiologia
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| Series: | Jornal Brasileiro de Pneumologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132017000200095&lng=en&tlng=en |
| _version_ | 1819097580372492288 |
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| author | Rubia Rodrigues Clarice Rosa Olivo Juliana Dias Lourenço Alyne Riane Daniela Aparecida de Brito Cervilha Juliana Tiyaki Ito Milton de Arruda Martins Fernanda Degobbi Tenório Quirino dos Santos Lopes |
| author_facet | Rubia Rodrigues Clarice Rosa Olivo Juliana Dias Lourenço Alyne Riane Daniela Aparecida de Brito Cervilha Juliana Tiyaki Ito Milton de Arruda Martins Fernanda Degobbi Tenório Quirino dos Santos Lopes |
| author_sort | Rubia Rodrigues |
| collection | DOAJ |
| description | ABSTRACT Objective: To describe a murine model of emphysema induced by a combination of exposure to cigarette smoke (CS) and instillation of porcine pancreatic elastase (PPE). Methods: A total of 38 C57BL/6 mice were randomly divided into four groups: control (one intranasal instillation of 0.9% saline solution); PPE (two intranasal instillations of PPE); CS (CS exposure for 60 days); and CS + PPE (two intranasal instillations of PPE + CS exposure for 60 days). At the end of the experimental protocol, all animals were anesthetized and tracheostomized for calculation of respiratory mechanics parameters. Subsequently, all animals were euthanized and their lungs were removed for measurement of the mean linear intercept (Lm) and determination of the numbers of cells that were immunoreactive to macrophage (MAC)-2 antigen, matrix metalloproteinase (MMP)-12, and glycosylated 91-kDa glycoprotein (gp91phox) in the distal lung parenchyma and peribronchial region. Results: Although there were no differences among the four groups regarding the respiratory mechanics parameters assessed, there was an increase in the Lm in the CS + PPE group. The numbers of MAC-2-positive cells in the peribronchial region and distal lung parenchyma were higher in the CS + PPE group than in the other groups, as were the numbers of cells that were positive for MMP-12 and gp91phox, although only in the distal lung parenchyma. Conclusions: Our model of emphysema induced by a combination of PPE instillation and CS exposure results in a significant degree of parenchymal destruction in a shorter time frame than that employed in other models of CS-induced emphysema, reinforcing the importance of protease-antiprotease imbalance and oxidant-antioxidant imbalance in the pathogenesis of emphysema. |
| first_indexed | 2024-12-22T00:17:21Z |
| format | Article |
| id | doaj.art-303f96f806a0459faf29f0166cacad7b |
| institution | Directory Open Access Journal |
| issn | 1806-3756 |
| language | English |
| last_indexed | 2024-12-22T00:17:21Z |
| publisher | Sociedade Brasileira de Pneumologia e Tisiologia |
| record_format | Article |
| series | Jornal Brasileiro de Pneumologia |
| spelling | doaj.art-303f96f806a0459faf29f0166cacad7b2022-12-21T18:45:16ZengSociedade Brasileira de Pneumologia e TisiologiaJornal Brasileiro de Pneumologia1806-37564329510010.1590/s1806-37562016000000179S1806-37132017000200095A murine model of elastase- and cigarette smoke-induced emphysemaRubia RodriguesClarice Rosa OlivoJuliana Dias LourençoAlyne RianeDaniela Aparecida de Brito CervilhaJuliana Tiyaki ItoMilton de Arruda MartinsFernanda Degobbi Tenório Quirino dos Santos LopesABSTRACT Objective: To describe a murine model of emphysema induced by a combination of exposure to cigarette smoke (CS) and instillation of porcine pancreatic elastase (PPE). Methods: A total of 38 C57BL/6 mice were randomly divided into four groups: control (one intranasal instillation of 0.9% saline solution); PPE (two intranasal instillations of PPE); CS (CS exposure for 60 days); and CS + PPE (two intranasal instillations of PPE + CS exposure for 60 days). At the end of the experimental protocol, all animals were anesthetized and tracheostomized for calculation of respiratory mechanics parameters. Subsequently, all animals were euthanized and their lungs were removed for measurement of the mean linear intercept (Lm) and determination of the numbers of cells that were immunoreactive to macrophage (MAC)-2 antigen, matrix metalloproteinase (MMP)-12, and glycosylated 91-kDa glycoprotein (gp91phox) in the distal lung parenchyma and peribronchial region. Results: Although there were no differences among the four groups regarding the respiratory mechanics parameters assessed, there was an increase in the Lm in the CS + PPE group. The numbers of MAC-2-positive cells in the peribronchial region and distal lung parenchyma were higher in the CS + PPE group than in the other groups, as were the numbers of cells that were positive for MMP-12 and gp91phox, although only in the distal lung parenchyma. Conclusions: Our model of emphysema induced by a combination of PPE instillation and CS exposure results in a significant degree of parenchymal destruction in a shorter time frame than that employed in other models of CS-induced emphysema, reinforcing the importance of protease-antiprotease imbalance and oxidant-antioxidant imbalance in the pathogenesis of emphysema.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132017000200095&lng=en&tlng=enTabacoModelos animaisEnfisemaFenômenos fisiológicos respiratóriosLesão pulmonar |
| spellingShingle | Rubia Rodrigues Clarice Rosa Olivo Juliana Dias Lourenço Alyne Riane Daniela Aparecida de Brito Cervilha Juliana Tiyaki Ito Milton de Arruda Martins Fernanda Degobbi Tenório Quirino dos Santos Lopes A murine model of elastase- and cigarette smoke-induced emphysema Jornal Brasileiro de Pneumologia Tabaco Modelos animais Enfisema Fenômenos fisiológicos respiratórios Lesão pulmonar |
| title | A murine model of elastase- and cigarette smoke-induced emphysema |
| title_full | A murine model of elastase- and cigarette smoke-induced emphysema |
| title_fullStr | A murine model of elastase- and cigarette smoke-induced emphysema |
| title_full_unstemmed | A murine model of elastase- and cigarette smoke-induced emphysema |
| title_short | A murine model of elastase- and cigarette smoke-induced emphysema |
| title_sort | murine model of elastase and cigarette smoke induced emphysema |
| topic | Tabaco Modelos animais Enfisema Fenômenos fisiológicos respiratórios Lesão pulmonar |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132017000200095&lng=en&tlng=en |
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