Selection and Experimental Evaluation of Universal Primers to Study the Fungal Microbiome of Higher Plants

The impact of primer choice on results of metabarcoding studies was experimentally evaluated by analyzing fungal communities associated with leaves of four plant species. Significant differences in target specificity of primers were highlighted by a percentage of plant reads ranging from almost noth...

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Bibliographic Details
Main Authors: Silvia Scibetta, Leonardo Schena, Ahmed Abdelfattah, Sonia Pangallo, Santa O. Cacciola
Format: Article
Language:English
Published: The American Phytopathological Society 2019-02-01
Series:Phytobiomes Journal
Subjects:
Online Access:https://apsjournals.apsnet.org/doi/full/10.1094/PBIOMES-02-18-0009-R