Systematic Functional and Computational Analysis of Glucose-Binding Residues in Glycoside Hydrolase Family GH116

Systematic Functional and Computational Analysis of Glucose-Binding Residues in Glycoside Hydrolase Family GH116

Glycoside hydrolases (GH) bind tightly to the sugar moiety at the glycosidic bond being hydrolyzed to stabilize its transition state conformation. We endeavored to assess the importance of glucose-binding residues in GH family 116 (GH116) β-glucosidases, which include human β-glucosylceramidase 2 (G...

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Bibliographic Details
Main Authors: Meng Huang, Salila Pengthaisong, Ratana Charoenwattanasatien, Natechanok Thinkumrob, Jitrayut Jitonnom, James R. Ketudat Cairns
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Catalysts
Subjects:
β-glucosidase
glucose-binding residues
mutagenesis
enzyme kinetics
X-ray crystallography
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Online Access:https://www.mdpi.com/2073-4344/12/3/343

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https://www.mdpi.com/2073-4344/12/3/343

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