Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell...

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Bibliographic Details
Main Authors: María Questa, Leonardo Romorini, Carolina Blüguermann, Claudia María Solari, Gabriel Neiman, Carlos Luzzani, María Élida Scassa, Gustavo Emilio Sevlever, Alejandra Sonia Guberman, Santiago Gabriel Miriuka
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506115002068

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http://www.sciencedirect.com/science/article/pii/S1873506115002068

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