Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-03-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506115002068 |
| _version_ | 1818328176183476224 |
|---|---|
| author | María Questa Leonardo Romorini Carolina Blüguermann Claudia María Solari Gabriel Neiman Carlos Luzzani María Élida Scassa Gustavo Emilio Sevlever Alejandra Sonia Guberman Santiago Gabriel Miriuka |
| author_facet | María Questa Leonardo Romorini Carolina Blüguermann Claudia María Solari Gabriel Neiman Carlos Luzzani María Élida Scassa Gustavo Emilio Sevlever Alejandra Sonia Guberman Santiago Gabriel Miriuka |
| author_sort | María Questa |
| collection | DOAJ |
| description | Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. |
| first_indexed | 2024-12-13T12:28:00Z |
| format | Article |
| id | doaj.art-30607d771dbc4c7099d923c5f9fedae8 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-13T12:28:00Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-30607d771dbc4c7099d923c5f9fedae82022-12-21T23:46:10ZengElsevierStem Cell Research1873-50611876-77532016-03-0116230030310.1016/j.scr.2015.12.026Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblastsMaría Questa0Leonardo Romorini1Carolina Blüguermann2Claudia María Solari3Gabriel Neiman4Carlos Luzzani5María Élida Scassa6Gustavo Emilio Sevlever7Alejandra Sonia Guberman8Santiago Gabriel Miriuka9LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaLIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaLIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaLaboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, C1428EGA, Buenos Aires, ArgentinaLIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaLIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaLIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaLIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaLaboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, C1428EGA, Buenos Aires, ArgentinaLIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, ArgentinaHuman foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.http://www.sciencedirect.com/science/article/pii/S1873506115002068 |
| spellingShingle | María Questa Leonardo Romorini Carolina Blüguermann Claudia María Solari Gabriel Neiman Carlos Luzzani María Élida Scassa Gustavo Emilio Sevlever Alejandra Sonia Guberman Santiago Gabriel Miriuka Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts Stem Cell Research |
| title | Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts |
| title_full | Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts |
| title_fullStr | Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts |
| title_full_unstemmed | Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts |
| title_short | Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts |
| title_sort | generation of ipsc line ipsc fh2 1 in hypoxic conditions from human foreskin fibroblasts |
| url | http://www.sciencedirect.com/science/article/pii/S1873506115002068 |
| work_keys_str_mv | AT mariaquesta generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT leonardoromorini generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT carolinabluguermann generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT claudiamariasolari generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT gabrielneiman generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT carlosluzzani generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT mariaelidascassa generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT gustavoemiliosevlever generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT alejandrasoniaguberman generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts AT santiagogabrielmiriuka generationofipsclineipscfh21inhypoxicconditionsfromhumanforeskinfibroblasts |