Sequence Analysis of Putative potB, potC, and potD Genes from Serratia rubidae
Amplification of putative potBCD genes from Serratia rubidae was conducted by PCR using a pair of primers FERC and RERC. A fragment with ~1800 bp size was ligated to pGEM-T Easy vector then cloned to competent Escherichia coli DH5α. The recombinant plasmid was sequenced using SP6, T7 and two inter...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Indonesian Society for Microbiology
2010-08-01
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Series: | Microbiology Indonesia |
Subjects: | |
Online Access: | https://jurnal.permi.or.id/index.php/mionline/article/view/105 |