Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair
Adequate vascularization is crucial for the repair of bone defects. Platelet-derived growth factor-BB (PDGF-BB) has been shown to promote H-type vessel formation, a specialized blood vessel, strongly positive for CD31 and endomucin, coupling angiogenesis and osteogenesis. However, the lack of a cont...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127523008225 |
| _version_ | 1797526239826673664 |
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| author | Jianguo Wei Dongming Duan Yihan Jing Shuai Huang Jiamin Liu Aiju Lou Tianhua Xiao Yapei Chen Xia Chen Yuwei Zeng Song Liu Zhao Wang Yixi Yang Lei Zhou Zenghui Wu Le Wang |
| author_facet | Jianguo Wei Dongming Duan Yihan Jing Shuai Huang Jiamin Liu Aiju Lou Tianhua Xiao Yapei Chen Xia Chen Yuwei Zeng Song Liu Zhao Wang Yixi Yang Lei Zhou Zenghui Wu Le Wang |
| author_sort | Jianguo Wei |
| collection | DOAJ |
| description | Adequate vascularization is crucial for the repair of bone defects. Platelet-derived growth factor-BB (PDGF-BB) has been shown to promote H-type vessel formation, a specialized blood vessel, strongly positive for CD31 and endomucin, coupling angiogenesis and osteogenesis. However, the lack of a controllable growth factor delivery limits its clinical application, causing certain complications associated with PDGF-BB administration. This study developed an injectable heparin-conjugated hydrogel (GHH) for controlled delivery to facilitate the effective binding of PDGF-BB and rapid vascularized bone regeneration. Herein, in vitro experiments involving the controlled release of PDGF-BB showed that the PDGF-BB-loaded GHH (GHHP) hydrogel demonstrated excellent cytocompatibility and osteogenic properties. The subcutaneous implantation model in rats revealed that the GHHP hydrogel has the potential to induce the formation of H-type blood vessels in vivo. Furthermore, the healing of distal femoral condyle defects in rat models in vivo demonstrated that GHHP could substantially promote H-type angiogenesis and effectively repair bone defects. Our injectable heparin-conjugated sustained-release PDGF-BB hydrogel has excellent angiogenic and osteogenic properties, thus providing a novel and promising solution for bone defect repair. |
| first_indexed | 2024-03-10T09:27:22Z |
| format | Article |
| id | doaj.art-3082e002e2cf4e7a893418223df79bf4 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-10T09:27:22Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-3082e002e2cf4e7a893418223df79bf42023-11-22T04:46:22ZengElsevierMaterials & Design0264-12752023-11-01235112407Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repairJianguo Wei0Dongming Duan1Yihan Jing2Shuai Huang3Jiamin Liu4Aiju Lou5Tianhua Xiao6Yapei Chen7Xia Chen8Yuwei Zeng9Song Liu10Zhao Wang11Yixi Yang12Lei Zhou13Zenghui Wu14Le Wang15Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Second Affiliated Hospital of Guangzhou Medical University, 250 Changgang East Road, Haizhu District, Guangzhou 510260, PR ChinaGuangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaTranslational Research Centre of Regenerative Medicine and 3D Printing of Guangzhou Medical University, Guangdong Province Engineering Research Center for Biomedical Engineering, State Key Laboratory of Respiratory Disease, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaDepartment of Orthopaedic Surgery, The Second Affiliated Hospital of Guangzhou Medical University, 250 Changgang East Road, Haizhu District, Guangzhou 510260, PR ChinaGuangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaDepartment of Rheumatology, Liwan Central Hospital of Guangzhou, 35 Liwan Road, Liwan District, Guangzhou 510030, PR ChinaGuangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaDepartment of Ultrasound Medicine, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaGuangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaDepartment of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaGuangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaGuangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR ChinaGuangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Corresponding authors at: Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, P.R. China.Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Corresponding authors at: Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, P.R. China.Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Department of Orthopaedic Surgery, The Third Affiliated Hospital of Guangzhou Medical University 63 Duobao Road, Liwan District, Guangzhou 510150, PR China; Corresponding authors at: Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Liwan District, Guangzhou 510150, P.R. China.Adequate vascularization is crucial for the repair of bone defects. Platelet-derived growth factor-BB (PDGF-BB) has been shown to promote H-type vessel formation, a specialized blood vessel, strongly positive for CD31 and endomucin, coupling angiogenesis and osteogenesis. However, the lack of a controllable growth factor delivery limits its clinical application, causing certain complications associated with PDGF-BB administration. This study developed an injectable heparin-conjugated hydrogel (GHH) for controlled delivery to facilitate the effective binding of PDGF-BB and rapid vascularized bone regeneration. Herein, in vitro experiments involving the controlled release of PDGF-BB showed that the PDGF-BB-loaded GHH (GHHP) hydrogel demonstrated excellent cytocompatibility and osteogenic properties. The subcutaneous implantation model in rats revealed that the GHHP hydrogel has the potential to induce the formation of H-type blood vessels in vivo. Furthermore, the healing of distal femoral condyle defects in rat models in vivo demonstrated that GHHP could substantially promote H-type angiogenesis and effectively repair bone defects. Our injectable heparin-conjugated sustained-release PDGF-BB hydrogel has excellent angiogenic and osteogenic properties, thus providing a novel and promising solution for bone defect repair.http://www.sciencedirect.com/science/article/pii/S0264127523008225Platelet derived growth factor-BBInjectable hydrogelsFemoral condyle defectH-type blood vesselsVascularized bone regeneration |
| spellingShingle | Jianguo Wei Dongming Duan Yihan Jing Shuai Huang Jiamin Liu Aiju Lou Tianhua Xiao Yapei Chen Xia Chen Yuwei Zeng Song Liu Zhao Wang Yixi Yang Lei Zhou Zenghui Wu Le Wang Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair Materials & Design Platelet derived growth factor-BB Injectable hydrogels Femoral condyle defect H-type blood vessels Vascularized bone regeneration |
| title | Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair |
| title_full | Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair |
| title_fullStr | Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair |
| title_full_unstemmed | Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair |
| title_short | Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair |
| title_sort | heparin conjugated injectable hydrogels with sustained releasing capability for promotion of h type vessels formation and rat femoral bone defects repair |
| topic | Platelet derived growth factor-BB Injectable hydrogels Femoral condyle defect H-type blood vessels Vascularized bone regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S0264127523008225 |
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