Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels
Obesity is associated with an accelerated aging process, which prevents healthy aging. Both obesity and aging were manifested in the peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) level. These studies fulfill the scientific gap in assembled pharmacological activity assay of Caul...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.939073/full |
| _version_ | 1811292158969249792 |
|---|---|
| author | Happy Kurnia Permatasari Fahrul Nurkolis Hardinsyah Hardinsyah Nurpudji Astuti Taslim Nindy Sabrina Faisal Maulana Ibrahim Jodi Visnu Dian Aruni Kumalawati Sri Awalia Febriana Toto Sudargo Melvin Junior Tanner Isma Kurniatanty Vincentius Mario Yusuf Ronald Rompies Muhammad Rahimi Bahar Holipah Holipah Nelly Mayulu |
| author_facet | Happy Kurnia Permatasari Fahrul Nurkolis Hardinsyah Hardinsyah Nurpudji Astuti Taslim Nindy Sabrina Faisal Maulana Ibrahim Jodi Visnu Dian Aruni Kumalawati Sri Awalia Febriana Toto Sudargo Melvin Junior Tanner Isma Kurniatanty Vincentius Mario Yusuf Ronald Rompies Muhammad Rahimi Bahar Holipah Holipah Nelly Mayulu |
| author_sort | Happy Kurnia Permatasari |
| collection | DOAJ |
| description | Obesity is associated with an accelerated aging process, which prevents healthy aging. Both obesity and aging were manifested in the peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) level. These studies fulfill the scientific gap in assembled pharmacological activity assay of Caulerpa racemosa done in a previous preclinical trial. Six major compounds from sea grape (C. racemosa) extract were evaluated using an in silico approach against human pancreatic lipase, a-glucosidase, and a-amylase to predict prospective anti-obesity candidates. The lipase inhibitory activity of the extract reached 90.30 ± 0.40%, 1.75% lower than orlistat. The a-amylase inhibitory assay of the extract was 84.07 ± 5.28%, while the inhibitory activity against a-glucosidase was 81.67 ± 1.54%; both were lower than acarbose. We observe the effect of C. racemosa extract as anti-obesity with anti-aging by evaluating the obesity parameters in the human body for a 4-week period. There was a significant decrease in blood glucose, total cholesterol, low-density lipoprotein (LDL), triglycerides (TG), waist circumference, waist-hip ratio, and body weight (p < 0.05); PGC-1α and high-density lipoprotein (HDL) increased significantly (p = 0.000), in Group B when compared with Group A. Our study revealed that sea grape extract is a potent anti-obesity with an anti-aging reagent that does not produce any significant adverse effects. |
| first_indexed | 2024-04-13T04:41:15Z |
| format | Article |
| id | doaj.art-308331cace1b49c69e00c1cd9e10cce9 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T04:41:15Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-308331cace1b49c69e00c1cd9e10cce92022-12-22T03:01:58ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-07-01910.3389/fnut.2022.939073939073Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α LevelsHappy Kurnia Permatasari0Fahrul Nurkolis1Hardinsyah Hardinsyah2Nurpudji Astuti Taslim3Nindy Sabrina4Faisal Maulana Ibrahim5Jodi Visnu6Dian Aruni Kumalawati7Sri Awalia Febriana8Toto Sudargo9Melvin Junior Tanner10Isma Kurniatanty11Vincentius Mario Yusuf12Ronald Rompies13Muhammad Rahimi Bahar14Holipah Holipah15Nelly Mayulu16Department of Biochemistry and Biomolecular, Faculty of Medicine, Brawijaya University, Malang, IndonesiaDepartment of Biological Sciences, Sunan Kalijaga State Islamic University, Yogyakarta, IndonesiaDepartment of Applied Nutrition, IPB University, Bogor, IndonesiaDepartment of Clinical Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, IndonesiaDepartment of Nutrition, Dietetics and Food, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, VIC, AustraliaPharmaceutical Analysis and Medicinal Chemistry, Universitas Padjadjaran, Sumedang, IndonesiaFaculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, IndonesiaDepartment of Biological Sciences, Sunan Kalijaga State Islamic University, Yogyakarta, IndonesiaFaculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, IndonesiaFaculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, IndonesiaDepartment of Nutrition, Faculty of Public Health, University of Indonesia, Depok, IndonesiaDepartment of Biological Sciences, Sunan Kalijaga State Islamic University, Yogyakarta, IndonesiaFaculty of Medicine, Universitas Brawijaya, Malang, Indonesia0Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia0Faculty of Medicine, Sam Ratulangi University, Manado, IndonesiaFaculty of Medicine, Universitas Brawijaya, Malang, Indonesia0Faculty of Medicine, Sam Ratulangi University, Manado, IndonesiaObesity is associated with an accelerated aging process, which prevents healthy aging. Both obesity and aging were manifested in the peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) level. These studies fulfill the scientific gap in assembled pharmacological activity assay of Caulerpa racemosa done in a previous preclinical trial. Six major compounds from sea grape (C. racemosa) extract were evaluated using an in silico approach against human pancreatic lipase, a-glucosidase, and a-amylase to predict prospective anti-obesity candidates. The lipase inhibitory activity of the extract reached 90.30 ± 0.40%, 1.75% lower than orlistat. The a-amylase inhibitory assay of the extract was 84.07 ± 5.28%, while the inhibitory activity against a-glucosidase was 81.67 ± 1.54%; both were lower than acarbose. We observe the effect of C. racemosa extract as anti-obesity with anti-aging by evaluating the obesity parameters in the human body for a 4-week period. There was a significant decrease in blood glucose, total cholesterol, low-density lipoprotein (LDL), triglycerides (TG), waist circumference, waist-hip ratio, and body weight (p < 0.05); PGC-1α and high-density lipoprotein (HDL) increased significantly (p = 0.000), in Group B when compared with Group A. Our study revealed that sea grape extract is a potent anti-obesity with an anti-aging reagent that does not produce any significant adverse effects.https://www.frontiersin.org/articles/10.3389/fnut.2022.939073/fullPGC-1αobesityCaulerpa racemosanutraceuticalslipid profile |
| spellingShingle | Happy Kurnia Permatasari Fahrul Nurkolis Hardinsyah Hardinsyah Nurpudji Astuti Taslim Nindy Sabrina Faisal Maulana Ibrahim Jodi Visnu Dian Aruni Kumalawati Sri Awalia Febriana Toto Sudargo Melvin Junior Tanner Isma Kurniatanty Vincentius Mario Yusuf Ronald Rompies Muhammad Rahimi Bahar Holipah Holipah Nelly Mayulu Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels Frontiers in Nutrition PGC-1α obesity Caulerpa racemosa nutraceuticals lipid profile |
| title | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_full | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_fullStr | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_full_unstemmed | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_short | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_sort | metabolomic assay computational screening and pharmacological evaluation of caulerpa racemosa as an anti obesity with anti aging by altering lipid profile and peroxisome proliferator activated receptor γ coactivator 1 α levels |
| topic | PGC-1α obesity Caulerpa racemosa nutraceuticals lipid profile |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.939073/full |
| work_keys_str_mv | AT happykurniapermatasari metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT fahrulnurkolis metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT hardinsyahhardinsyah metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT nurpudjiastutitaslim metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT nindysabrina metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT faisalmaulanaibrahim metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT jodivisnu metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT dianarunikumalawati metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT sriawaliafebriana metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT totosudargo metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT melvinjuniortanner metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT ismakurniatanty metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT vincentiusmarioyusuf metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT ronaldrompies metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT muhammadrahimibahar metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT holipahholipah metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels AT nellymayulu metabolomicassaycomputationalscreeningandpharmacologicalevaluationofcaulerparacemosaasanantiobesitywithantiagingbyalteringlipidprofileandperoxisomeproliferatoractivatedreceptorgcoactivator1alevels |