Comparative Study of DNA Extraction Methods for the PCR Detection of Intestinal Parasites in Human Stool Samples

Comparative Study of DNA Extraction Methods for the PCR Detection of Intestinal Parasites in Human Stool Samples

Stool samples typically contain PCR inhibitors; however, helminths are difficult to lyse and can cause false-negative PCR results. We assessed the effective methods for extracting DNA from different kinds of intestinal parasites. We compared the most common DNA extraction methods from stool samples,...

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Bibliographic Details
Main Authors: Siriporn Srirungruang, Buraya Mahajindawong, Panachai Nimitpanya, Uthaitip Bunkasem, Pattama Ayuyoe, Surang Nuchprayoon, Vivornpun Sanprasert
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Diagnostics
Subjects:
DNA extraction
bead-beating procedure
stool samples
intestinal parasites
polymerase chain reaction
PCR inhibitor
Online Access:https://www.mdpi.com/2075-4418/12/11/2588

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https://www.mdpi.com/2075-4418/12/11/2588

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