Homology-independent targeted insertion (HITI) enables guided CAR knock-in and efficient clinical scale CAR-T cell manufacturing

Homology-independent targeted insertion (HITI) enables guided CAR knock-in and efficient clinical scale CAR-T cell manufacturing

Abstract Background Chimeric Antigen Receptor (CAR) T cells are now standard of care (SOC) for some patients with B cell and plasma cell malignancies and could disrupt the therapeutic landscape of solid tumors. However, access to CAR-T cells is not adequate to meet clinical needs, in part due to hig...

Full description

Bibliographic Details
Main Authors: Hyatt Balke-Want, Vimal Keerthi, Nikolaos Gkitsas, Andrew G. Mancini, Gavin L. Kurgan, Carley Fowler, Peng Xu, Xikun Liu, Kyle Asano, Sunny Patel, Christopher J. Fisher, Annie K. Brown, Ramya H. Tunuguntla, Shabnum Patel, Elena Sotillo, Crystal L. Mackall, Steven A. Feldman
Format: Article
Language:English
Published: BMC 2023-06-01
Series:Molecular Cancer
Subjects:
CRISPR/Cas9
Homology-independent targeted insertion (HITI)
Targeted insertion
Non-viral CAR-T cell
GMP
Genomic safety
Online Access:https://doi.org/10.1186/s12943-023-01799-7

Internet

https://doi.org/10.1186/s12943-023-01799-7

Similar Items