Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicity
Excess extracellular glutamate leads to excitotoxicity, which induces neuronal death through the overactivation of N-methyl-D-aspartate receptors (NMDARs). Excitotoxicity is thought to be closely related to various acute and chronic neurological disorders, such as stroke and Alzheimer’s disease. Pol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=1;spage=178;epage=184;aulast=Sun |
| _version_ | 1819142506612260864 |
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| author | Chong Sun Xin-Cheng Cao Zhi-Yang Liu Chao-Lin Ma Bao-Ming Li |
| author_facet | Chong Sun Xin-Cheng Cao Zhi-Yang Liu Chao-Lin Ma Bao-Ming Li |
| author_sort | Chong Sun |
| collection | DOAJ |
| description | Excess extracellular glutamate leads to excitotoxicity, which induces neuronal death through the overactivation of N-methyl-D-aspartate receptors (NMDARs). Excitotoxicity is thought to be closely related to various acute and chronic neurological disorders, such as stroke and Alzheimer’s disease. Polygalasaponin F (PGSF) is a triterpenoid saponin monomer that can be isolated from Polygala japonica, and has been reported to protect cells against apoptosis. To investigate the mechanisms underlying the neuroprotective effects of PGSF against glutamate-induced cytotoxicity, PGSF-pretreated hippocampal neurons were exposed to glutamate for 24 hours. The results demonstrated that PGSF inhibited glutamate-induced hippocampal neuron death in a concentration-dependent manner and reduced glutamate-induced Ca2+ overload in the cultured neurons. In addition, PGSF partially blocked the excess activity of NMDARs, inhibited both the downregulation of NMDAR subunit NR2A expression and the upregulation of NMDAR subunit NR2B expression, and upregulated the expression of phosphorylated cyclic adenosine monophosphate-responsive element-binding protein and brain-derived neurotrophic factor. These findings suggest that PGSF protects cultured hippocampal neurons against glutamate-induced cytotoxicity by regulating NMDARs. The study was approved by the Institutional Animal Care Committee of Nanchang University (approval No. 2017-0006) on December 29, 2017. |
| first_indexed | 2024-12-22T12:11:26Z |
| format | Article |
| id | doaj.art-30c279d255ef42a483bb4456dd7b3d82 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-22T12:11:26Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-30c279d255ef42a483bb4456dd7b3d822022-12-21T18:26:17ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-0117117818410.4103/1673-5374.314321Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicityChong SunXin-Cheng CaoZhi-Yang LiuChao-Lin MaBao-Ming LiExcess extracellular glutamate leads to excitotoxicity, which induces neuronal death through the overactivation of N-methyl-D-aspartate receptors (NMDARs). Excitotoxicity is thought to be closely related to various acute and chronic neurological disorders, such as stroke and Alzheimer’s disease. Polygalasaponin F (PGSF) is a triterpenoid saponin monomer that can be isolated from Polygala japonica, and has been reported to protect cells against apoptosis. To investigate the mechanisms underlying the neuroprotective effects of PGSF against glutamate-induced cytotoxicity, PGSF-pretreated hippocampal neurons were exposed to glutamate for 24 hours. The results demonstrated that PGSF inhibited glutamate-induced hippocampal neuron death in a concentration-dependent manner and reduced glutamate-induced Ca2+ overload in the cultured neurons. In addition, PGSF partially blocked the excess activity of NMDARs, inhibited both the downregulation of NMDAR subunit NR2A expression and the upregulation of NMDAR subunit NR2B expression, and upregulated the expression of phosphorylated cyclic adenosine monophosphate-responsive element-binding protein and brain-derived neurotrophic factor. These findings suggest that PGSF protects cultured hippocampal neurons against glutamate-induced cytotoxicity by regulating NMDARs. The study was approved by the Institutional Animal Care Committee of Nanchang University (approval No. 2017-0006) on December 29, 2017.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=1;spage=178;epage=184;aulast=Sunbdnf; ca2+ homeostasis; excitotoxicity; glutamate; hippocampal neurons; pcreb; polygalasaponin f; neuroprotection; nr2a; nr2b |
| spellingShingle | Chong Sun Xin-Cheng Cao Zhi-Yang Liu Chao-Lin Ma Bao-Ming Li Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicity Neural Regeneration Research bdnf; ca2+ homeostasis; excitotoxicity; glutamate; hippocampal neurons; pcreb; polygalasaponin f; neuroprotection; nr2a; nr2b |
| title | Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicity |
| title_full | Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicity |
| title_fullStr | Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicity |
| title_full_unstemmed | Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicity |
| title_short | Polygalasaponin F protects hippocampal neurons against glutamate-induced cytotoxicity |
| title_sort | polygalasaponin f protects hippocampal neurons against glutamate induced cytotoxicity |
| topic | bdnf; ca2+ homeostasis; excitotoxicity; glutamate; hippocampal neurons; pcreb; polygalasaponin f; neuroprotection; nr2a; nr2b |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=1;spage=178;epage=184;aulast=Sun |
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