The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus Infections
In 2009, the co-circulation of H5N1 and H1N1pdm09 raised concerns that a reassortment event may lead to highly pathogenic influenza strains. H1N1pdm09 and H5N1 are able to infect the same target cells of the lower respiratory tract. To investigate the capacity of the emergence of reassortant viruses...
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MDPI AG
2018-09-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/9/504 |
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| author | Olivier Ferraris Jean-Sébastien Casalegno Emilie Frobert Maude Bouscambert Duchamp Martine Valette Frédéric Jacquot Hervé Raoul Bruno Lina Michèle Ottmann |
| author_facet | Olivier Ferraris Jean-Sébastien Casalegno Emilie Frobert Maude Bouscambert Duchamp Martine Valette Frédéric Jacquot Hervé Raoul Bruno Lina Michèle Ottmann |
| author_sort | Olivier Ferraris |
| collection | DOAJ |
| description | In 2009, the co-circulation of H5N1 and H1N1pdm09 raised concerns that a reassortment event may lead to highly pathogenic influenza strains. H1N1pdm09 and H5N1 are able to infect the same target cells of the lower respiratory tract. To investigate the capacity of the emergence of reassortant viruses, we characterized viruses obtained from the co-infection of cells with H5N1 (A/Turkey/13/2006) and H1N1pdm09 (A/Lyon/969/2009 H1N1). In our analysis, all the screened reassortants possessed the PB2, HA, and NP segments from H5N1 and acquired one or two of the H1N1pdm09 segments. Moreover, the in vivo infections showed that the acquisition of the NS segment from H1N1pdm09 increased the virulence of H5N1 in mice. We conclude, therefore, that reassortment can occur between these two viruses, even if this process has never been detected in nature. |
| first_indexed | 2024-12-13T09:39:16Z |
| format | Article |
| id | doaj.art-30ced1804fda4d2b9afad7b8836b3009 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T09:39:16Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-30ced1804fda4d2b9afad7b8836b30092022-12-21T23:52:15ZengMDPI AGViruses1999-49152018-09-0110950410.3390/v10090504v10090504The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus InfectionsOlivier Ferraris0Jean-Sébastien Casalegno1Emilie Frobert2Maude Bouscambert Duchamp3Martine Valette4Frédéric Jacquot5Hervé Raoul6Bruno Lina7Michèle Ottmann8Laboratoire de Virologie et Pathologies Humaines Virpath, CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, 69372 CEDEX 08 Lyon, FranceLaboratoire de Virologie et Pathologies Humaines Virpath, CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, 69372 CEDEX 08 Lyon, FranceLaboratoire de Virologie et Pathologies Humaines Virpath, CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, 69372 CEDEX 08 Lyon, FranceLaboratoire de Virologie, Institut des Agents Infectieux, Groupement Hospitalier Nord des Hospices Civils de Lyon, 69317 CEDEX 04 Lyon, FranceLaboratoire de Virologie, Institut des Agents Infectieux, Groupement Hospitalier Nord des Hospices Civils de Lyon, 69317 CEDEX 04 Lyon, FranceLaboratoire P4 Jean Mérieux Inserm US003, 69365 CEDEX 07 Lyon, FranceLaboratoire P4 Jean Mérieux Inserm US003, 69365 CEDEX 07 Lyon, FranceLaboratoire de Virologie et Pathologies Humaines Virpath, CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, 69372 CEDEX 08 Lyon, FranceLaboratoire de Virologie et Pathologies Humaines Virpath, CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm U1111, CNRS UMR5308, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, 69372 CEDEX 08 Lyon, FranceIn 2009, the co-circulation of H5N1 and H1N1pdm09 raised concerns that a reassortment event may lead to highly pathogenic influenza strains. H1N1pdm09 and H5N1 are able to infect the same target cells of the lower respiratory tract. To investigate the capacity of the emergence of reassortant viruses, we characterized viruses obtained from the co-infection of cells with H5N1 (A/Turkey/13/2006) and H1N1pdm09 (A/Lyon/969/2009 H1N1). In our analysis, all the screened reassortants possessed the PB2, HA, and NP segments from H5N1 and acquired one or two of the H1N1pdm09 segments. Moreover, the in vivo infections showed that the acquisition of the NS segment from H1N1pdm09 increased the virulence of H5N1 in mice. We conclude, therefore, that reassortment can occur between these two viruses, even if this process has never been detected in nature.http://www.mdpi.com/1999-4915/10/9/504virusinfluenzainfluenza AH5N1H1N1pdmreassortmentpathogenicitymouse model |
| spellingShingle | Olivier Ferraris Jean-Sébastien Casalegno Emilie Frobert Maude Bouscambert Duchamp Martine Valette Frédéric Jacquot Hervé Raoul Bruno Lina Michèle Ottmann The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus Infections Viruses virus influenza influenza A H5N1 H1N1pdm reassortment pathogenicity mouse model |
| title | The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus Infections |
| title_full | The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus Infections |
| title_fullStr | The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus Infections |
| title_full_unstemmed | The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus Infections |
| title_short | The NS Segment of H1N1pdm09 Enhances H5N1 Pathogenicity in a Mouse Model of Influenza Virus Infections |
| title_sort | ns segment of h1n1pdm09 enhances h5n1 pathogenicity in a mouse model of influenza virus infections |
| topic | virus influenza influenza A H5N1 H1N1pdm reassortment pathogenicity mouse model |
| url | http://www.mdpi.com/1999-4915/10/9/504 |
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