Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage
The purpose of this study was to investigate the effects of inoculation with two lactic acid bacteria (LAB) strains (Lacticaseibacillus rhamnosus and Lentilactobacillus buchneri) and the addition of four corn flour proportions (0, 3, 6, and 9%) in different treatments, on the composition and functio...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.904193/full |
| _version_ | 1798002068849426432 |
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| author | Changrong Wu Wentao Sun Yuan Huang Sheng Dai Chao Peng Yulong Zheng Chao Chen Jun Hao |
| author_facet | Changrong Wu Wentao Sun Yuan Huang Sheng Dai Chao Peng Yulong Zheng Chao Chen Jun Hao |
| author_sort | Changrong Wu |
| collection | DOAJ |
| description | The purpose of this study was to investigate the effects of inoculation with two lactic acid bacteria (LAB) strains (Lacticaseibacillus rhamnosus and Lentilactobacillus buchneri) and the addition of four corn flour proportions (0, 3, 6, and 9%) in different treatments, on the composition and function of the bacterial community in whole-plant paper mulberry silage. The different treatments promoted Lactiplantibacillus, Lentilactobacillus, and Lacticaseibacillus growth, but the microbial species responsible for fermentation differed among the treatments. High species diversity and various Gram-negative bacteria, such as Flavitalea sp., Pantoea agglomerans, Acinetobacter pittii, Turicibacter sanguinis, and Ralstonia pickettii, were found in the uninoculated LAB treatments. A beneficial bacterium, Lactobacillus johnsonii, was discovered for the first time in whole-plant paper mulberry silage. LAB inoculation simplifies the microbial community structure, and beneficial Lactobacillus as a key species aggregates in the inoculated treatment group. However, L. rhamnosus inoculation alone may have limited bacteriostatic activity against in whole-plant paper mulberry silage. Compared with silage lacking corn flour, amino sugar and nucleotide sugar metabolism, galactose metabolism, the phosphotransferase system and the pentose phosphate pathway metabolic activity were increased in corn flour-containing silage. Whole-plant paper mulberry can be used as a high-quality silage to provide high-quality feed resources for sustainable ruminant livestock production. Moreover, additive use is necessary for preparing paper mulberry silage. |
| first_indexed | 2024-04-11T11:46:20Z |
| format | Article |
| id | doaj.art-30d262fd76ef4764a09622a3ef87453a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T11:46:20Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-30d262fd76ef4764a09622a3ef87453a2022-12-22T04:25:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.904193904193Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silageChangrong WuWentao SunYuan HuangSheng DaiChao PengYulong ZhengChao ChenJun HaoThe purpose of this study was to investigate the effects of inoculation with two lactic acid bacteria (LAB) strains (Lacticaseibacillus rhamnosus and Lentilactobacillus buchneri) and the addition of four corn flour proportions (0, 3, 6, and 9%) in different treatments, on the composition and function of the bacterial community in whole-plant paper mulberry silage. The different treatments promoted Lactiplantibacillus, Lentilactobacillus, and Lacticaseibacillus growth, but the microbial species responsible for fermentation differed among the treatments. High species diversity and various Gram-negative bacteria, such as Flavitalea sp., Pantoea agglomerans, Acinetobacter pittii, Turicibacter sanguinis, and Ralstonia pickettii, were found in the uninoculated LAB treatments. A beneficial bacterium, Lactobacillus johnsonii, was discovered for the first time in whole-plant paper mulberry silage. LAB inoculation simplifies the microbial community structure, and beneficial Lactobacillus as a key species aggregates in the inoculated treatment group. However, L. rhamnosus inoculation alone may have limited bacteriostatic activity against in whole-plant paper mulberry silage. Compared with silage lacking corn flour, amino sugar and nucleotide sugar metabolism, galactose metabolism, the phosphotransferase system and the pentose phosphate pathway metabolic activity were increased in corn flour-containing silage. Whole-plant paper mulberry can be used as a high-quality silage to provide high-quality feed resources for sustainable ruminant livestock production. Moreover, additive use is necessary for preparing paper mulberry silage.https://www.frontiersin.org/articles/10.3389/fmicb.2022.904193/fullwhole-plant paper mulberrylactic acid bacteriabacterial communitysilage qualityfunctional prediction |
| spellingShingle | Changrong Wu Wentao Sun Yuan Huang Sheng Dai Chao Peng Yulong Zheng Chao Chen Jun Hao Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage Frontiers in Microbiology whole-plant paper mulberry lactic acid bacteria bacterial community silage quality functional prediction |
| title | Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage |
| title_full | Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage |
| title_fullStr | Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage |
| title_full_unstemmed | Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage |
| title_short | Effects of different additives on the bacterial community and fermentation mode of whole-plant paper mulberry silage |
| title_sort | effects of different additives on the bacterial community and fermentation mode of whole plant paper mulberry silage |
| topic | whole-plant paper mulberry lactic acid bacteria bacterial community silage quality functional prediction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.904193/full |
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