miR-92b-3p靶向肌细胞增强子2D抑制心肌细胞肥大
【目的】探讨微小RNA microRNA-92b-3p（miR-92b-3p）对心肌细胞肥大的调控作用及其作用靶基因。【方法】建 立血管紧张素Ⅱ（Ang-Ⅱ）诱导的C57BL/6小鼠心肌肥厚模型。通过尾静脉注射胆固醇修饰的miR-92b-3p模拟物（agomiR- 92b-3p）来增加小鼠心肌中miR-92b-3p水平，分别设立3个实验组：agomiR-negative control （agomiR-NC）+saline组，agomiR- NC+Ang-Ⅱ组，agomiR-92b-3p+Ang-Ⅱ组。用Ang-Ⅱ诱导乳小鼠心肌细胞建立心肌细胞肥大模型；双荧光素酶报告基因实验 检测mi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2017-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43567819/ |