Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing module
Summary: Cellular regulation of pH is crucial for internal biological processes and for the import and export of ions and nutrients. In the yeast Saccharomyces cerevisiae, the major proton pump (Pma1) is regulated by glucose. Glucose is also an inhibitor of the energy sensor Snf1/AMPK, which is cons...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222013554 |
| _version_ | 1798033553149132800 |
|---|---|
| author | Kobi J. Simpson-Lavy Martin Kupiec |
| author_facet | Kobi J. Simpson-Lavy Martin Kupiec |
| author_sort | Kobi J. Simpson-Lavy |
| collection | DOAJ |
| description | Summary: Cellular regulation of pH is crucial for internal biological processes and for the import and export of ions and nutrients. In the yeast Saccharomyces cerevisiae, the major proton pump (Pma1) is regulated by glucose. Glucose is also an inhibitor of the energy sensor Snf1/AMPK, which is conserved in all eukaryotes. Here, we demonstrate that a poly-histidine (polyHIS) tract in the pre-kinase region (PKR) of Snf1 functions as a pH-sensing module (PSM) and regulates Snf1 activity. This regulation is independent from, and unaffected by, phosphorylation at T210, the major regulatory control of Snf1, but is controlled by the Pma1 plasma-membrane proton pump. By examining the PKR from additional yeast species, and by varying the number of histidines in the PKR, we determined that the polyHIS functions progressively. This regulation mechanism links the activity of a key enzyme with the metabolic status of the cell at any given moment. |
| first_indexed | 2024-04-11T20:32:16Z |
| format | Article |
| id | doaj.art-313810acec7647d9b4b58f3e55f2b4b2 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-11T20:32:16Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-313810acec7647d9b4b58f3e55f2b4b22022-12-22T04:04:29ZengElsevieriScience2589-00422022-10-012510105083Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing moduleKobi J. Simpson-Lavy0Martin Kupiec1The Shmunis School of Biomedicine & Cancer Research, Tel Aviv University, Ramat Aviv 69978, IsraelThe Shmunis School of Biomedicine & Cancer Research, Tel Aviv University, Ramat Aviv 69978, Israel; Corresponding authorSummary: Cellular regulation of pH is crucial for internal biological processes and for the import and export of ions and nutrients. In the yeast Saccharomyces cerevisiae, the major proton pump (Pma1) is regulated by glucose. Glucose is also an inhibitor of the energy sensor Snf1/AMPK, which is conserved in all eukaryotes. Here, we demonstrate that a poly-histidine (polyHIS) tract in the pre-kinase region (PKR) of Snf1 functions as a pH-sensing module (PSM) and regulates Snf1 activity. This regulation is independent from, and unaffected by, phosphorylation at T210, the major regulatory control of Snf1, but is controlled by the Pma1 plasma-membrane proton pump. By examining the PKR from additional yeast species, and by varying the number of histidines in the PKR, we determined that the polyHIS functions progressively. This regulation mechanism links the activity of a key enzyme with the metabolic status of the cell at any given moment.http://www.sciencedirect.com/science/article/pii/S2589004222013554Biological sciencesbiochemistrymolecular biology |
| spellingShingle | Kobi J. Simpson-Lavy Martin Kupiec Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing module iScience Biological sciences biochemistry molecular biology |
| title | Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing module |
| title_full | Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing module |
| title_fullStr | Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing module |
| title_full_unstemmed | Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing module |
| title_short | Regulation of yeast Snf1 (AMPK) by a polyhistidine containing pH sensing module |
| title_sort | regulation of yeast snf1 ampk by a polyhistidine containing ph sensing module |
| topic | Biological sciences biochemistry molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222013554 |
| work_keys_str_mv | AT kobijsimpsonlavy regulationofyeastsnf1ampkbyapolyhistidinecontainingphsensingmodule AT martinkupiec regulationofyeastsnf1ampkbyapolyhistidinecontainingphsensingmodule |