Identification of Suitable Internal Control miRNAs in Bovine Milk Small Extracellular Vesicles for Normalization in Quantitative Real-Time Polymerase Chain Reaction

Identification of Suitable Internal Control miRNAs in Bovine Milk Small Extracellular Vesicles for Normalization in Quantitative Real-Time Polymerase Chain Reaction

This study aimed to identify a suitable RNA extraction kit and stable internal control microRNA (miRNA) in bovine milk small extracellular vesicles (sEVs) for a quantitative polymerase chain reaction (qPCR) analysis. Two RNA extraction kits, miRNeasy Micro Kit, and Maxwell RSC miRNA Tissue Kit, were...

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Bibliographic Details
Main Authors: Md. Matiur Rahman, Ryoka Nakanishi, Fumi Tsukada, Shigeo Takashima, Yoshiko Wakihara, Yuji O. Kamatari, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Membranes
Subjects:
bovine milk small extracellular vesicles
internal control microRNA
normalization in quantitative polymerase chain reaction
RNA extraction kit
Online Access:https://www.mdpi.com/2077-0375/13/2/185

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https://www.mdpi.com/2077-0375/13/2/185

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