Efficient isolation of Magnolia protoplasts and the application to subcellular localization of MdeHSF1
Abstract Background Magnolia is a woody ornamental plant, which is widely used in urban landscaping. However, its lengthy juvenile period and recalcitrance to regeneration impedes functional characterization of its genes. Results We developed an efficient protoplast isolation and transient expressio...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2017-05-01
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Series: | Plant Methods |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13007-017-0193-3 |