In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells

Summary: State of the art techniques have been developed to isolate and analyze cells from various tissues, aiming to capture their in vivo state. However, the majority of cell isolation protocols involve lengthy mechanical and enzymatic dissociation steps followed by flow cytometry, exposing cells...

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Bibliographic Details
Main Authors: Léo Machado, Joana Esteves de Lima, Odile Fabre, Caroline Proux, Rachel Legendre, Anikó Szegedi, Hugo Varet, Lars Roed Ingerslev, Romain Barrès, Frédéric Relaix, Philippos Mourikis
Format: Article
Language:English
Published: Elsevier 2017-11-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717315437